Effects of Collagen Membranes and Bone Substitute Differ in Periodontal Ligament Cell Microtissues and Monolayers

Overview

Journal J Periodontol

Date 2021 Jul 5

PMID 34223638

Citations 7

Authors

Klara Janjic

Hermann Agis

Andreas Moritz

Xiaohui Rausch-Fan

Oleh Andrukhov

Affiliations

Soon will be listed here.

Abstract

Background: Barrier membranes and bone substitute are major tools of guided tissue regeneration (GTR) after periodontal disease. Integrity of the periodontal ligament plays a key role in periodontal health, but its functionality fails to be fully re-established by GTR after disease or trauma. Microtissue models suggest an in vivo-like model to develop novel GTR approaches due to its three-dimensionality. This study aims to assess the effects of collagen membranes and bone substitute on cell viability, adhesion and gene expression of regenerative and inflammatory biomarkers by periodontal ligament cell (PDLC) microtissues.

Methods: Human PDLC microtissues and monolayers were cultured on collagen membranes or bone substitute. After 24 hours incubation, metabolic activity, focal adhesion, mRNA and protein production of collagen-type-I (COL1A1), periostin (POSTN), vascular endothelial growth factor (VEGF), angiogenin (ANG), interleukin (IL)6 and IL8 were measured by resazurin-based toxicity assay, focal adhesion staining, quantitative polymerase chain reaction and enzyme-linked immunosorbent assay, respectively.

Results: PDLC microtissues and monolayers were viable on collagen membranes and bone substitute, but microtissues were less metabolically active. Dominant staining of actin filaments was found in PDLC microtissues on collagen membranes. COL1A1, POSTN, VEGF, ANG and IL6 were modulated in PDLC microtissues on bone substitute, while there were no significant changes on collagen membranes. PDLC monolayers showed a different character of gene expression changes.

Conclusions: PDLC microtissues and monolayers react diversely to collagen membranes and bone substitute. Further descriptive and mechanistic tests will be required to clarify the potential of PDLC microtissues as in vivo-like model for GTR.

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References

Sculean A, Nikolidakis D, Nikou G, Ivanovic A, Chapple I, Stavropoulos A . Biomaterials for promoting periodontal regeneration in human intrabony defects: a systematic review. Periodontol 2000. 2015; 68(1):182-216. DOI: 10.1111/prd.12086. View

Rothamel D, Benner M, Fienitz T, Happe A, Kreppel M, Nickenig H . Biodegradation pattern and tissue integration of native and cross-linked porcine collagen soft tissue augmentation matrices - an experimental study in the rat. Head Face Med. 2014; 10:10. PMC: 3984020. DOI: 10.1186/1746-160X-10-10. View

Edmondson R, Broglie J, Adcock A, Yang L . Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors. Assay Drug Dev Technol. 2014; 12(4):207-18. PMC: 4026212. DOI: 10.1089/adt.2014.573. View

Seetharaman S, Etienne-Manneville S . Microtubules at focal adhesions - a double-edged sword. J Cell Sci. 2019; 132(19). DOI: 10.1242/jcs.232843. View

Yamato H, Sanui T, Yotsumoto K, Nakao Y, Watanabe Y, Hayashi C . Combined application of geranylgeranylacetone and amelogenin promotes angiogenesis and wound healing in human periodontal ligament cells. J Cell Biochem. 2021; 122(7):716-730. DOI: 10.1002/jcb.29903. View

Mucino-Olmos E, Vazquez-Jimenez A, de Leon U, Matadamas-Guzman M, Maldonado V, Lopez-Santaella T . Unveiling functional heterogeneity in breast cancer multicellular tumor spheroids through single-cell RNA-seq. Sci Rep. 2020; 10(1):12728. PMC: 7391783. DOI: 10.1038/s41598-020-69026-7. View

Torricelli P, Fini M, Giavaresi G, Botter R, Beruto D, Giardino R . Biomimetic PMMA-based bone substitutes: a comparative in vitro evaluation of the effects of pulsed electromagnetic field exposure. J Biomed Mater Res A. 2002; 64(1):182-8. DOI: 10.1002/jbm.a.10372. View

Fukuba S, Okada M, Nohara K, Iwata T . Alloplastic Bone Substitutes for Periodontal and Bone Regeneration in Dentistry: Current Status and Prospects. Materials (Basel). 2021; 14(5). PMC: 7956697. DOI: 10.3390/ma14051096. View

Kojima T, Amizuka N, Suzuki A, Freitas P, Yoshizawa M, Kudo A . Histological examination of bone regeneration achieved by combining grafting with hydroxyapatite and thermoplastic bioresorbable plates. J Bone Miner Metab. 2007; 25(6):361-73. DOI: 10.1007/s00774-007-0763-y. View

10.

Janjic K, Agis H, Moritz A, Rausch-Fan X, Andrukhov O . Effects of collagen membranes and bone substitute differ in periodontal ligament cell microtissues and monolayers. J Periodontol. 2021; 93(5):697-708. PMC: 9291292. DOI: 10.1002/JPER.21-0225. View

11.

Albuquerque-Souza E, Schulte F, Chen T, Hardt M, Hasturk H, Van Dyke T . Maresin-1 and Resolvin E1 Promote Regenerative Properties of Periodontal Ligament Stem Cells Under Inflammatory Conditions. Front Immunol. 2020; 11:585530. PMC: 7546375. DOI: 10.3389/fimmu.2020.585530. View

12.

Blatt S, Thiem D, Pabst A, Al-Nawas B, Kammerer P . Does Platelet-Rich Fibrin Enhance the Early Angiogenetic Potential of Different Bone Substitute Materials? An In Vitro and In Vivo Analysis. Biomedicines. 2021; 9(1). PMC: 7827266. DOI: 10.3390/biomedicines9010061. View

13.

Zaidel-Bar R, Cohen M, Addadi L, Geiger B . Hierarchical assembly of cell-matrix adhesion complexes. Biochem Soc Trans. 2004; 32(Pt3):416-20. DOI: 10.1042/BST0320416. View

14.

Camelo M, Nevins M, Schenk R, Simion M, Rasperini G, Lynch S . Clinical, radiographic, and histologic evaluation of human periodontal defects treated with Bio-Oss and Bio-Gide. Int J Periodontics Restorative Dent. 2003; 18(4):321-31. View

15.

Lagies S, Schlimpert M, Neumann S, Waldin A, Kammerer B, Borner C . Cells grown in three-dimensional spheroids mirror in vivo metabolic response of epithelial cells. Commun Biol. 2020; 3(1):246. PMC: 7237469. DOI: 10.1038/s42003-020-0973-6. View

16.

Akilbekova D, Bratlie K . Quantitative Characterization of Collagen in the Fibrotic Capsule Surrounding Implanted Polymeric Microparticles through Second Harmonic Generation Imaging. PLoS One. 2015; 10(6):e0130386. PMC: 4488378. DOI: 10.1371/journal.pone.0130386. View

17.

. Position Paper: Periodontal Regeneration. J Periodontol. 2018; 76(9):1601-1622. DOI: 10.1902/jop.2005.76.9.1601. View

18.

Florjanski W, Orzeszek S, Olchowy A, Grychowska N, Wieckiewicz W, Malysa A . Modifications of Polymeric Membranes Used in Guided Tissue and Bone Regeneration. Polymers (Basel). 2019; 11(5). PMC: 6572646. DOI: 10.3390/polym11050782. View

19.

Williams D . There is no such thing as a biocompatible material. Biomaterials. 2014; 35(38):10009-14. DOI: 10.1016/j.biomaterials.2014.08.035. View

20.

Elgali I, Turri A, Xia W, Norlindh B, Johansson A, Dahlin C . Guided bone regeneration using resorbable membrane and different bone substitutes: Early histological and molecular events. Acta Biomater. 2015; 29:409-423. DOI: 10.1016/j.actbio.2015.10.005. View