Synthesis and Toxicity of Graphene Oxide Nanoparticles: A Literature Review of and Studies

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2021 Jul 5

PMID 34222470

Citations 43

Authors

Asmaa Rhazouani

Halima Gamrani

Mounir El Achaby

Khalid Aziz

Lhoucine Gebrati

Md Sahab Uddin

Faissal Aziz

Affiliations

Soon will be listed here.

Abstract

Nanomaterials have been widely used in many fields in the last decades, including electronics, biomedicine, cosmetics, food processing, buildings, and aeronautics. The application of these nanomaterials in the medical field could improve diagnosis, treatment, and prevention techniques. Graphene oxide (GO), an oxidized derivative of graphene, is currently used in biotechnology and medicine for cancer treatment, drug delivery, and cellular imaging. Also, GO is characterized by various physicochemical properties, including nanoscale size, high surface area, and electrical charge. However, the toxic effect of GO on living cells and organs is a limiting factor that limits its use in the medical field. Recently, numerous studies have evaluated the biocompatibility and toxicity of GO and . In general, the severity of this nanomaterial's toxic effects varies according to the administration route, the dose to be administered, the method of GO synthesis, and its physicochemical properties. This review brings together studies on the method of synthesis and structure of GO, characterization techniques, and physicochemical properties. Also, we rely on the toxicity of GO in cellular models and biological systems. Moreover, we mention the general mechanism of its toxicity.

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References

Mendonca M, Soares E, de Jesus M, Ceragioli H, Ferreira M, Catharino R . Reduced graphene oxide induces transient blood-brain barrier opening: an in vivo study. J Nanobiotechnology. 2015; 13:78. PMC: 4628296. DOI: 10.1186/s12951-015-0143-z. View

Lv M, Zhang Y, Liang L, Wei M, Hu W, Li X . Effect of graphene oxide on undifferentiated and retinoic acid-differentiated SH-SY5Y cells line. Nanoscale. 2012; 4(13):3861-6. DOI: 10.1039/c2nr30407d. View

Chen Y, Hu X, Sun J, Zhou Q . Specific nanotoxicity of graphene oxide during zebrafish embryogenesis. Nanotoxicology. 2015; 10(1):42-52. DOI: 10.3109/17435390.2015.1005032. View

Arbo M, Altknecht L, Cattani S, Braga W, Peruzzi C, Cestonaro L . In vitro cardiotoxicity evaluation of graphene oxide. Mutat Res Genet Toxicol Environ Mutagen. 2019; 841:8-13. DOI: 10.1016/j.mrgentox.2019.03.004. View

Chen M, Yin J, Liang Y, Yuan S, Wang F, Song M . Oxidative stress and immunotoxicity induced by graphene oxide in zebrafish. Aquat Toxicol. 2016; 174:54-60. DOI: 10.1016/j.aquatox.2016.02.015. View

Zhang Y, Ali S, Dervishi E, Xu Y, Li Z, Casciano D . Cytotoxicity effects of graphene and single-wall carbon nanotubes in neural phaeochromocytoma-derived PC12 cells. ACS Nano. 2010; 4(6):3181-6. DOI: 10.1021/nn1007176. View

Yang K, Gong H, Shi X, Wan J, Zhang Y, Liu Z . In vivo biodistribution and toxicology of functionalized nano-graphene oxide in mice after oral and intraperitoneal administration. Biomaterials. 2013; 34(11):2787-95. DOI: 10.1016/j.biomaterials.2013.01.001. View

Akhavan O, Ghaderi E . Toxicity of graphene and graphene oxide nanowalls against bacteria. ACS Nano. 2010; 4(10):5731-6. DOI: 10.1021/nn101390x. View

Han S, Kim J, Shin J, Hwang J, Lee J, Kim T . Pulmonary Responses of Sprague-Dawley Rats in Single Inhalation Exposure to Graphene Oxide Nanomaterials. Biomed Res Int. 2015; 2015:376756. PMC: 4534591. DOI: 10.1155/2015/376756. View

10.

Novoselov K, Geim A, Morozov S, Jiang D, Zhang Y, Dubonos S . Electric field effect in atomically thin carbon films. Science. 2004; 306(5696):666-9. DOI: 10.1126/science.1102896. View

11.

Singh S, Singh M, Kulkarni P, Sonkar V, Gracio J, Dash D . Amine-modified graphene: thrombo-protective safer alternative to graphene oxide for biomedical applications. ACS Nano. 2012; 6(3):2731-40. DOI: 10.1021/nn300172t. View

12.

Duch M, Budinger G, Liang Y, Soberanes S, Urich D, Chiarella S . Minimizing oxidation and stable nanoscale dispersion improves the biocompatibility of graphene in the lung. Nano Lett. 2011; 11(12):5201-7. PMC: 3237757. DOI: 10.1021/nl202515a. View

13.

Patlolla A, Randolph J, Kumari S, Tchounwou P . Toxicity Evaluation of Graphene Oxide in Kidneys of Sprague-Dawley Rats. Int J Environ Res Public Health. 2016; 13(4):380. PMC: 4847042. DOI: 10.3390/ijerph13040380. View

14.

Wang A, Pu K, Dong B, Liu Y, Zhang L, Zhang Z . Role of surface charge and oxidative stress in cytotoxicity and genotoxicity of graphene oxide towards human lung fibroblast cells. J Appl Toxicol. 2013; 33(10):1156-64. DOI: 10.1002/jat.2877. View

15.

Hu L, Fu Y, Rong L, Yang X, Li Y, Wang L . Evaluating the cytotoxicity of graphene oxide using embryonic stem cells-derived cells. J Biomed Mater Res A. 2020; 108(6):1321-1328. DOI: 10.1002/jbm.a.36904. View

16.

Yan H, Tao X, Yang Z, Li K, Yang H, Li A . Effects of the oxidation degree of graphene oxide on the adsorption of methylene blue. J Hazard Mater. 2014; 268:191-8. DOI: 10.1016/j.jhazmat.2014.01.015. View

17.

Chung C, Kim Y, Shin D, Ryoo S, Hong B, Min D . Biomedical applications of graphene and graphene oxide. Acc Chem Res. 2013; 46(10):2211-24. DOI: 10.1021/ar300159f. View

18.

Liang Y, Chen B, Li M, He J, Yin Z, Guo B . Injectable Antimicrobial Conductive Hydrogels for Wound Disinfection and Infectious Wound Healing. Biomacromolecules. 2020; 21(5):1841-1852. DOI: 10.1021/acs.biomac.9b01732. View

19.

Ferrari A, Basko D . Raman spectroscopy as a versatile tool for studying the properties of graphene. Nat Nanotechnol. 2013; 8(4):235-46. DOI: 10.1038/nnano.2013.46. View

20.

Wang Y, Li Z, Hu D, Lin C, Li J, Lin Y . Aptamer/graphene oxide nanocomplex for in situ molecular probing in living cells. J Am Chem Soc. 2010; 132(27):9274-6. DOI: 10.1021/ja103169v. View