Concentration- and Time-Dependent Dietary Exposure to Graphene Oxide and Silver Nanoparticles: Effects on Food Consumption and Assimilation, Digestive Enzyme Activities, and Body Mass in

Overview

Journal Insects

Specialty Biology

Date 2024 Feb 23

PMID 38392509

Authors

Reyhaneh Seyed Alian

Barbara Flasz

Andrzej Kedziorski

Lukasz Majchrzycki

Maria Augustyniak

Affiliations

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Abstract

The advancement of nanotechnology poses a real risk of insect exposure to nanoparticles (NPs) that can enter the digestive system through contaminated food or nanopesticides. This study examines whether the exposure of model insect species--to increasing graphene oxide (GO) and silver nanoparticle (AgNP) concentrations (2, 20, and 200 ppm and 4, 40, and 400 ppm, respectively) could change its digestive functions: enzymes' activities, food consumption, and assimilation. We noticed more pronounced alterations following exposure to AgNPs than to GO. They included increased activity of α-amylase, α-glucosidase, and lipase but inhibited protease activity. Prolonged exposure to higher concentrations of AgNPs resulted in a significantly decreased food consumption and changed assimilation compared with the control in adult crickets. A increase in body weight was observed in the insects from the Ag4 group and a decrease in body weight or no effects were observed in crickets from the Ag40 and Ag400 groups (i.e., 4, 40, or 400 ppm of AgNPs, respectively), suggesting that even a moderate disturbance in nutrient and energy availability may affect the body weight of an organism and its overall condition. This study underscores the intricate interplay between NPs and digestive enzymes, emphasizing the need for further investigation to comprehend the underlying mechanisms and consequences of these interactions.

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References

Zeng L, Zhu Z, Sun D . Novel graphene oxide/polymer composite membranes for the food industry: structures, mechanisms and recent applications. Crit Rev Food Sci Nutr. 2022; 62(14):3705-3722. DOI: 10.1080/10408398.2022.2054937. View

Malakar A, Kanel S, Ray C, Snow D, Nadagouda M . Nanomaterials in the environment, human exposure pathway, and health effects: A review. Sci Total Environ. 2020; 759:143470. DOI: 10.1016/j.scitotenv.2020.143470. View

Cebadero-Dominguez O, Jos A, Camean A, Catunescu G . Hazard characterization of graphene nanomaterials in the frame of their food risk assessment: A review. Food Chem Toxicol. 2022; 164:113014. DOI: 10.1016/j.fct.2022.113014. View

McGillicuddy E, Murray I, Kavanagh S, Morrison L, Fogarty A, Cormican M . Silver nanoparticles in the environment: Sources, detection and ecotoxicology. Sci Total Environ. 2016; 575:231-246. DOI: 10.1016/j.scitotenv.2016.10.041. View

Brennan J, Kanaras A, Nativo P, Tshikhudo T, Rees C, Fernandez L . Enzymatic activity of lipase-nanoparticle conjugates and the digestion of lipid liquid crystalline assemblies. Langmuir. 2010; 26(16):13590-9. DOI: 10.1021/la1018604. View

Deshmukh S, Patil S, Mullani S, Delekar S . Silver nanoparticles as an effective disinfectant: A review. Mater Sci Eng C Mater Biol Appl. 2019; 97:954-965. PMC: 7127744. DOI: 10.1016/j.msec.2018.12.102. View

Zhou H, McClements D . Recent Advances in the Gastrointestinal Fate of Organic and Inorganic Nanoparticles in Foods. Nanomaterials (Basel). 2022; 12(7). PMC: 9000219. DOI: 10.3390/nano12071099. View

El-Samad L, El-Ashram S, Hussein H, Abdul-Aziz K, Radwan E, Bakr N . Time-delayed effects of a single application of AgNPs on structure of testes and functions in Blaps polychresta Forskal, 1775 (Coleoptera: Tenebrionidae). Sci Total Environ. 2021; 806(Pt 2):150644. DOI: 10.1016/j.scitotenv.2021.150644. View

Flasz B, Dziewiecka M, Kedziorski A, Tarnawska M, Augustyniak M . Vitellogenin expression, DNA damage, health status of cells and catalase activity in Acheta domesticus selected according to their longevity after graphene oxide treatment. Sci Total Environ. 2020; 737:140274. DOI: 10.1016/j.scitotenv.2020.140274. View

10.

Dabrowski B, Zuchowska A, Brzozka Z . Graphene oxide internalization into mammalian cells - a review. Colloids Surf B Biointerfaces. 2022; 221:112998. DOI: 10.1016/j.colsurfb.2022.112998. View

11.

McClements D, Xiao H . Is nano safe in foods? Establishing the factors impacting the gastrointestinal fate and toxicity of organic and inorganic food-grade nanoparticles. NPJ Sci Food. 2019; 1:6. PMC: 6548419. DOI: 10.1038/s41538-017-0005-1. View

12.

Szelei J, Woodring J, Goettel M, Duke G, Jousset F, Liu K . Susceptibility of North-American and European crickets to Acheta domesticus densovirus (AdDNV) and associated epizootics. J Invertebr Pathol. 2010; 106(3):394-9. DOI: 10.1016/j.jip.2010.12.009. View

13.

Wang L, Wu D, Yu Z, Huang S, Zhang J . Hormone-mediated multi- and trans-generational reproductive toxicities of 1-ethyl-3-methylimidazolium hexafluorophosphate on Caenorhabditis elegans. Sci Total Environ. 2022; 863:160958. DOI: 10.1016/j.scitotenv.2022.160958. View

14.

Tortella G, Rubilar O, Duran N, Diez M, Martinez M, Parada J . Silver nanoparticles: Toxicity in model organisms as an overview of its hazard for human health and the environment. J Hazard Mater. 2020; 390:121974. DOI: 10.1016/j.jhazmat.2019.121974. View

15.

Yadav S, Raman A, Meena H, Goswami A, Bhawna , Kumar V . An Update on Graphene Oxide: Applications and Toxicity. ACS Omega. 2022; 7(40):35387-35445. PMC: 9558614. DOI: 10.1021/acsomega.2c03171. View

16.

Dziewiecka M, Karpeta-Kaczmarek J, Augustyniak M, Rost-Roszkowska M . Short-term in vivo exposure to graphene oxide can cause damage to the gut and testis. J Hazard Mater. 2017; 328:80-89. DOI: 10.1016/j.jhazmat.2017.01.012. View

17.

Dziewiecka M, Pawlyta M, Majchrzycki L, Balin K, Barteczko S, Czerkawska M . The Structure-Properties-Cytotoxicity Interplay: A Crucial Pathway to Determining Graphene Oxide Biocompatibility. Int J Mol Sci. 2021; 22(10). PMC: 8161018. DOI: 10.3390/ijms22105401. View

18.

Khodaparast Z, van Gestel C, Papadiamantis A, Goncalves S, Lynch I, Loureiro S . Toxicokinetics of silver nanoparticles in the mealworm Tenebrio molitor exposed via soil or food. Sci Total Environ. 2021; 777:146071. DOI: 10.1016/j.scitotenv.2021.146071. View

19.

Ghulam A, Dos Santos O, Hazeem L, Pizzorno Backx B, Bououdina M, Bellucci S . Graphene Oxide (GO) Materials-Applications and Toxicity on Living Organisms and Environment. J Funct Biomater. 2022; 13(2). PMC: 9224660. DOI: 10.3390/jfb13020077. View

20.

Lv M, Zhu E, Su Y, Li Q, Li W, Zhao Y . Trypsin-gold nanoparticle conjugates: binding, enzymatic activity, and stability. Prep Biochem Biotechnol. 2009; 39(4):429-38. DOI: 10.1080/10826060903210024. View