Purification, Characterization, and Self-Assembly of the Polysaccharide from

Overview

Journal Foods

Specialty Biotechnology

Date 2021 Jul 2

PMID 34208119

Citations 5

Authors

Fengrui Zhang

Jun Zheng

Zeyu Li

Zixuan Cai

Fengqiao Wang

Dong Yang

Affiliations

Soon will be listed here.

Abstract

The major polysaccharide component from the stalk of (sP) was extracted and purified. Gel filtration chromatography purified sP exhibited a molecular weight of around 1.7 kDa, which was verified by MALDI-ToF-MS. The monosaccharide analysis revealed its composition as rhamnose: arabinose: galactose: glucose: mannose: fructose with a molar ratio of 0.03:2.46:3.71:3.35:1.00:9.93, respectively. The Congo-red assay indicated that there was no tertiary structure of this polysaccharide, however, it self-assembled into a homogenous nanoparticle with a diameter of ~600 nm as revealed by the dynamic light scattering measurement. The solution behavior of this polysaccharide was simulated. The association of this polysaccharide was both time dependent and concentration dependent. sP forms spherical particles spontaneously as time passes by, and when the sP concentration increased, the spherical particles increased their sizes and eventually merged into cylindrical micelles. The diversity of sP hydrodynamic behavior endowed potential versatility in its future applications.

Citing Articles

Efficient removal of allicin from the stalk of Allium fistulosum for dietary fiber production.

Li Y, Ma J, Cao Y, Yang D NPJ Sci Food. 2024; 8(1):32.

PMID: 38877017 PMC: 11178807. DOI: 10.1038/s41538-024-00275-w.

Dietary fiber chemical structure determined gut microbiota dynamics.

Meng X, Zheng J, Wang F, Zheng J, Yang D Imeta. 2024; 1(4):e64.

PMID: 38867894 PMC: 10989905. DOI: 10.1002/imt2.64.

Structural Characterization, In Vitro Digestion Property, and Biological Activity of Sweet Corn Cob Polysaccharide Iron (III) Complexes.

Xiu W, Wang X, Yu S, Na Z, Li C, Yang M Molecules. 2023; 28(7).

PMID: 37049724 PMC: 10096156. DOI: 10.3390/molecules28072961.

Natural Deep Eutectic Solvent-Assisted Extraction, Structural Characterization, and Immunomodulatory Activity of Polysaccharides from .

Wang Y, Xu F, Cheng J, Wu X, Xu J, Li C Molecules. 2022; 27(22).

PMID: 36432126 PMC: 9696976. DOI: 10.3390/molecules27228020.

Acceptability of Vegetable Fortified in Sub-Saharan Africa.

Cai Z, Meng X, Nyirenda D, Mandala W, Li X, Yang D Nutrients. 2021; 13(10).

PMID: 34684406 PMC: 8537100. DOI: 10.3390/nu13103405.

References

Rajaonarivony M, Vauthier C, Couarraze G, Puisieux F, Couvreur P . Development of a new drug carrier made from alginate. J Pharm Sci. 1993; 82(9):912-7. DOI: 10.1002/jps.2600820909. View

Barazani O, Dudai N, Khadka U, Golan-Goldhirsh A . Cadmium accumulation in Allium schoenoprasum L. grown in an aqueous medium. Chemosphere. 2004; 57(9):1213-8. DOI: 10.1016/j.chemosphere.2004.08.037. View

Kucekova Z, Mlcek J, Humpolicek P, Rop O, Valasek P, Saha P . Phenolic compounds from Allium schoenoprasum, Tragopogon pratensis and Rumex acetosa and their antiproliferative effects. Molecules. 2011; 16(11):9207-17. PMC: 6264378. DOI: 10.3390/molecules16119207. View

Sun Y, Liang H, Zhang X, Tong H, Liu J . Structural elucidation and immunological activity of a polysaccharide from the fruiting body of Armillaria mellea. Bioresour Technol. 2008; 100(5):1860-3. DOI: 10.1016/j.biortech.2008.09.036. View

Chen Y, Mao W, Wang B, Zhou L, Gu Q, Chen Y . Preparation and characterization of an extracellular polysaccharide produced by the deep-sea fungus Penicillium griseofulvum. Bioresour Technol. 2013; 132:178-81. DOI: 10.1016/j.biortech.2012.12.075. View

Smith P, Wang H, York W, Pena M, Urbanowicz B . Designer biomass for next-generation biorefineries: leveraging recent insights into xylan structure and biosynthesis. Biotechnol Biofuels. 2017; 10:286. PMC: 5708106. DOI: 10.1186/s13068-017-0973-z. View

Tong H, Xia F, Feng K, Sun G, Gao X, Sun L . Structural characterization and in vitro antitumor activity of a novel polysaccharide isolated from the fruiting bodies of Pleurotus ostreatus. Bioresour Technol. 2008; 100(4):1682-6. DOI: 10.1016/j.biortech.2008.09.004. View

Kapolna E, Fodor P . Bioavailability of selenium from selenium-enriched green onions (Allium fistulosum) and chives (Allium schoenoprasum) after 'in vitro' gastrointestinal digestion. Int J Food Sci Nutr. 2007; 58(4):282-96. DOI: 10.1080/09637480601154335. View

Stajner D, canadanovic-Brunet J, Pavlovic A . Allium schoenoprasum L., as a natural antioxidant. Phytother Res. 2004; 18(7):522-4. DOI: 10.1002/ptr.1472. View

10.

Luo X, Liu J, Zheng P, Li M, Zhou Y, Huang L . Promoting enzymatic hydrolysis of lignocellulosic biomass by inexpensive soy protein. Biotechnol Biofuels. 2019; 12:51. PMC: 6417190. DOI: 10.1186/s13068-019-1387-x. View

11.

Wang N, Wu X . Preparation and characterization of agarose hydrogel nanoparticles for protein and peptide drug delivery. Pharm Dev Technol. 1997; 2(2):135-42. DOI: 10.3109/10837459709022618. View

12.

Timite G, Mitaine-Offer A, Miyamoto T, Tanaka C, Mirjolet J, Duchamp O . Structure and cytotoxicity of steroidal glycosides from Allium schoenoprasum. Phytochemistry. 2013; 88:61-6. DOI: 10.1016/j.phytochem.2012.12.001. View

13.

Yu P, Zhou F, Yang D . Curdlan conformation change during its hydrolysis by multi-domain β-1,3-glucanases. Food Chem. 2019; 287:20-27. DOI: 10.1016/j.foodchem.2019.02.070. View

14.

Eisazadeh S, Asadi Kapourchal S, Homaee M, Noorhosseini S, Damalas C . Chive (Allium schoenoprasum L.) response as a phytoextraction plant in cadmium-contaminated soils. Environ Sci Pollut Res Int. 2018; 26(1):152-160. DOI: 10.1007/s11356-018-3545-2. View

15.

Zeng Y, Himmel M, Ding S . Visualizing chemical functionality in plant cell walls. Biotechnol Biofuels. 2017; 10:263. PMC: 5708085. DOI: 10.1186/s13068-017-0953-3. View

16.

Chen S, Han Y, Sun C, Dai L, Yang S, Wei Y . Effect of molecular weight of hyaluronan on zein-based nanoparticles: Fabrication, structural characterization and delivery of curcumin. Carbohydr Polym. 2018; 201:599-607. DOI: 10.1016/j.carbpol.2018.08.116. View

17.

Hartig S, Greene R, DasGupta J, Carlesso G, Dikov M, Prokop A . Multifunctional nanoparticulate polyelectrolyte complexes. Pharm Res. 2007; 24(12):2353-69. DOI: 10.1007/s11095-007-9459-1. View

18.

Stajner D, Popovic B, Calic-Dragosavac D, Malencic D, Zdravkovic-Korac S . Comparative study on Allium schoenoprasum cultivated plant and Allium schoenoprasum tissue culture organs antioxidant status. Phytother Res. 2011; 25(11):1618-22. DOI: 10.1002/ptr.3394. View

19.

Goo B, Baek G, Choi D, Park Y, Synytsya A, Bleha R . Characterization of a renewable extracellular polysaccharide from defatted microalgae Dunaliella tertiolecta. Bioresour Technol. 2012; 129:343-50. DOI: 10.1016/j.biortech.2012.11.077. View

20.

Cao J, Lv Q, Zhang B, Chen H . Structural characterization and hepatoprotective activities of polysaccharides from the leaves of Toona sinensis (A. Juss) Roem. Carbohydr Polym. 2019; 212:89-101. DOI: 10.1016/j.carbpol.2019.02.031. View