Enzymatic Degradation of Multiple Major Mycotoxins by Dye-Decolorizing Peroxidase from

The co-occurrence of multiple mycotoxins, including aflatoxin B (AFB), zearalenone (ZEN) and deoxynivalenol (DON), widely exists in cereal-based animal feed and food. At present, most reported mycotoxins degrading enzymes target only a certain type of mycotoxins. Therefore, it is of great significance for mining enzymes involved in the simultaneous degradation of different types of mycotoxins. In this study, a dye-decolorizing peroxidase-encoding gene from SCK6 was cloned and expressed in BL21/pG-Tf2. The purified recombinant DyP was capable of oxidizing various substrates, including lignin phenolic model compounds 2,6-dimethylphenol and guaiacol, the substrate 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), anthraquinone dye reactive blue 19 and azo dye reactive black 5, as well as Mn. In addition, DyP could efficiently degrade different types of mycotoxins, including AFB, ZEN and DON, in presence of Mn. More important, the toxicities of their corresponding enzymatic degradation products AFB-diol, 15-OH-ZEN and CHO were significantly lower than AFB, ZEN and DON. In summary, these results proved that DyP was a promising candidate for the simultaneous degradation of multiple mycotoxins in animal feed and food.