Candidate L-methionine Target PiRNA Regulatory Networks Analysis Response to Cocaine-conditioned Place Preference in Mice

Overview

Journal Brain Behav

Specialty Psychology

Date 2021 Jul 1

PMID 34196487

Citations 3

Authors

Kunlin Zhang

Guanyu Ji

Mei Zhao

Yan Wang

Affiliations

Soon will be listed here.

Abstract

Background: Methionine has been proven to inhibit addictive behaviors of cocaine dependence. However, the mechanism of methionine response to cocaine CPP is unknown. Recent evidence highlights piRNAs to regulate genes via a miRNA-like mechanism. Here, next-generation sequencing is used to study mechanism on methionine response to drug-induced behaviors though piRNA.

Methods: l-methionine treatment cocaine CPP animal model was used to do non-coding RNA sequencing. There were four groups to sequence: saline+saline (SS), MET+saline (MS), MET+cocaine (MC), and cocaine+saline. Combining mRNA sequencing data, the network and regulation of piRNA were analyzed with their corresponding mRNA and miRNA.

Results: Analysis of the piRNAome reveals that piRNAs inversely regulated their target mRNA genes. KEGG analysis of DE-piRNA target mRNA genes were enriched in Morphine addiction, GABAergic synapse and Cholinergic synapse pathway. Furthermore, four significantly differential expressed genes Cacna2d3, Epha6, Nedd4l, and Vav2 were identified and regulated by piRNAs in the process of l-methionine inhibits cocaine CPP. Thereinto, Vav2 was regulated by multiple DE piRNAs by sharing the common sequence: GTCTCTCCAGCCACCTT. Meanwhile, it was found that piRNA positively regulates miRNA and three genes Bcl3, Il20ra, and Insrr were identified and regulated by piRNA through miRNA.

Conclusion: The results showed that piRNA negatively regulated target mRNA genes and positively regulated target miRNA genes. Genes located in substance dependence, signal transduction and also nervous functions pathways were identified. When taken together, these data may explain the roles of l-methionine in counteracting the effects of cocaine CPP via piRNAs.

Citing Articles

Substance Addiction Rehabilitation Drugs.

Yuan S, Jiang S, Zhang Z, Li Z, Hu J Pharmaceuticals (Basel). 2024; 17(5).

PMID: 38794185 PMC: 11124501. DOI: 10.3390/ph17050615.

Identification of miRNA-mediated gene regulatory networks in L-methionine exposure counteracts cocaine-conditioned place preference in mice.

Wang Y, Yang L, Zhou H, Zhang K, Zhao M Front Genet. 2023; 13:1076156.

PMID: 36744178 PMC: 9893020. DOI: 10.3389/fgene.2022.1076156.

Candidate l-methionine target piRNA regulatory networks analysis response to cocaine-conditioned place preference in mice.

Zhang K, Ji G, Zhao M, Wang Y Brain Behav. 2021; 11(8):e2272.

PMID: 34196487 PMC: 8413732. DOI: 10.1002/brb3.2272.

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