Synthesis, Characterisation, Crystal Structure and Antimicrobial Evaluation of Novel 6-alkoxyergosta-4,6,8(14),22-tetraen-3-one Derived from Natural Ergosta-5,7,22-trien-3β-ol

In this study, we report a facile transformation starting from 5α-hydroxyergosta-7,22-dien-3,6-dione to afford two novel compounds: 6-methoxyergosta-4,6,8(14),22-tetraen-3-one and 6-ethoxyergosta-4,6,8(14),22-tetraen-3-one ( using alcoholic acid catalysis. Their structures were elucidated using NMR experiments, FT-IR, MS and X-ray analysis. These compounds were evaluated for antibacterial activity using the disk and broth diffusion test. In those tests, compound was found to be the most significant antibacterial agent. In general, compounds showed inhibition zone in the range of 7.00-12.3 mm for and , meanwhile for Gram-negative bacteria and was found to be in the range of 7.00-8.00 mm. For the most active, compound , MIC was significantly lower than that reported for ergosterol, in a value of 160 µg/mL. Overall, these compounds were more active than their natural precursor.