Effect of Genistein Supplementation on the Progression of Neoplasms and the Level of the Modified Nucleosides in Rats With Mammary Cancer

Overview

Journal In Vivo

Specialty Oncology

Date 2021 Jun 28

PMID 34182481

Citations 4

Authors

Karolina Banys

Joanna Giebultowicz

Marcin Sobczak

Rafal Wyrebiak

Wojciech Bielecki

Robert Wrzesien

Barbara Bobrowska-Korczak

Affiliations

Soon will be listed here.

Abstract

Background/aim: The aim of the study was to assess the impact of nano-, micro-, and macro-sized-genistein on the growth and development of neoplasms in rats with mammary cancer. Additionally, the effect on the kinetics of changes (9-11-17-20 week of a rat's life) in the levels of methyl derivatives: 1-methyladenine, 3-methyladenine, 7-methylguanine, 1-methylguanine, 1-methyladenosine, 7-methylguanosine, O-methyl-guanosine and N6-methyl-2'-deoxyguanosine in the urine of rats was analyzed.

Materials And Methods: Female Sprague-Dawley rats divided into 4 groups were used in the study. Animals were fed only a control diet or diets supplemented with the nano-, micro- and macro-sized genistein. To induce the mammary adenocarcinoma, rats were treated with 7,12-dimethylbenz[a]anthracene (DMBA). Modified nucleosides were determined by a high-performance liquid chromatography coupled to mass spectrometry method (LC-MS/MS).

Results: The supplementation of the diet of animals with genistein resulted in an increase in the excretion of methylated derivatives in the urine of rats. In the animals receiving standard diet, the levels of methyl derivatives increased during the study or remained relatively low. In the case of animals whose diet was supplemented with the various forms of genistein, the levels of methylated derivatives were very high from the beginning.

Conclusion: High levels of methyl derivatives can influence carcinogenesis.

Citing Articles

Genistein Supplementation and Bone Health in Breast Cancer in Rats.

Skrajnowska D, Bielecki W, Szterk A, Ofiara K, Bobrowska-Korczak B Nutrients. 2024; 16(6).

PMID: 38542823 PMC: 10974594. DOI: 10.3390/nu16060912.

Inflammation Factors and Genistein Supplementation in Cancer-Preliminary Research.

Banys K, Jelinska M, Wrzosek M, Skrajnowska D, Wrzesien R, Bielecki W Curr Issues Mol Biol. 2024; 46(3):2166-2180.

PMID: 38534756 PMC: 10968949. DOI: 10.3390/cimb46030140.

Genistein anticancer efficacy during induced oral squamous cell carcinoma: an experimental study.

Hussein A, Attaai A, Zahran A J Egypt Natl Canc Inst. 2022; 34(1):37.

PMID: 36058937 DOI: 10.1186/s43046-022-00140-5.

The Effect of Genistein Supplementation on Cholesterol Oxidation Products and Fatty Acid Profiles in Serums of Rats with Breast Cancer.

Banys K, Stawarska A, Wyrebiak R, Bielecki W, Bobrowska-Korczak B Foods. 2022; 11(4).

PMID: 35206081 PMC: 8871115. DOI: 10.3390/foods11040605.

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