Using Genetically Encoded Calcium Indicators to Study Astrocyte Physiology: A Field Guide

Overview

Journal Front Cell Neurosci

Specialty Cell Biology

Date 2021 Jun 28

PMID 34177468

Citations 15

Authors

Christian Lohr

Antonia Beiersdorfer

Timo Fischer

Daniela Hirnet

Natalie Rotermund

Jessica Sauer

Kristina Schulz

Christine E Gee

Affiliations

Soon will be listed here.

Abstract

Ca imaging is the most frequently used technique to study glial cell physiology. While chemical Ca indicators served to visualize and measure changes in glial cell cytosolic Ca concentration for several decades, genetically encoded Ca indicators (GECIs) have become state of the art in recent years. Great improvements have been made since the development of the first GECI and a large number of GECIs with different physical properties exist, rendering it difficult to select the optimal Ca indicator. This review discusses some of the most frequently used GECIs and their suitability for glial cell research.

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