The Multispecific Thyroid Hormone Transporter OATP1C1 Mediates Cell-specific Sulforhodamine 101-labeling of Hippocampal Astrocytes

Overview

Journal Brain Struct Funct

Specialty Neurology

Date 2013 Oct 17

PMID 24129767

Citations 38

Authors

Christian Schnell

Ali Shahmoradi

Sven P Wichert

Steffen Mayerl

Yohannes Hagos

Heike Heuer

Moritz J Rossner

Swen Hulsmann

Affiliations

Soon will be listed here.

Abstract

Sulforhodamine 101 (SR101) is widely used for astrocyte identification, though the labeling mechanism remains unknown and the efficacy of labeling in different brain regions is heterogeneous. By combining region-specific isolation of astrocytes followed by transcriptome analysis, two-photon excitation microscopy, and mouse genetics, we identified the thyroid hormone transporter OATP1C1 as the SR101-uptake transporter in hippocampus and cortex.

Citing Articles

Spontaneous Calcium Transients Recorded from Striatal Astrocytes in a Preclinical Model of Autism.

Saavedra-Bonilla H, Varman D, Reyes-Haro D Neurochem Res. 2024; 49(11):3069-3077.

PMID: 39120794 PMC: 11450070. DOI: 10.1007/s11064-024-04218-5.

Analysis of Functional NMDA Receptors in Astrocytes.

Kirchhoff F, Tang W Methods Mol Biol. 2024; 2799:201-223.

PMID: 38727909 DOI: 10.1007/978-1-0716-3830-9_11.

Angiotensin II increases respiratory rhythmic activity in the preBötzinger complex without inducing astroglial calcium signaling.

Tacke C, Bischoff A, Harb A, Vafadari B, Hulsmann S Front Cell Neurosci. 2023; 17:1111263.

PMID: 36816850 PMC: 9932970. DOI: 10.3389/fncel.2023.1111263.

Contributions of Astrocyte and Neuronal Volume to CA1 Neuron Excitability Changes in Elevated Extracellular Potassium.

Walch E, Bilas A, Bebawy V, Lam A, Murphy T, Sriram S Front Cell Neurosci. 2022; 16:930384.

PMID: 35936495 PMC: 9352931. DOI: 10.3389/fncel.2022.930384.

Targeting gliovascular connexins prevents inflammatory blood-brain barrier leakage and astrogliosis.

De Bock M, De Smet M, Verwaerde S, Tahiri H, Schumacher S, Van Haver V JCI Insight. 2022; 7(16).

PMID: 35881483 PMC: 9462469. DOI: 10.1172/jci.insight.135263.

References

Thompson R, Jackson M, Olah M, Rungta R, Hines D, Beazely M . Activation of pannexin-1 hemichannels augments aberrant bursting in the hippocampus. Science. 2008; 322(5907):1555-9. DOI: 10.1126/science.1165209. View

Nimmerjahn A, Kirchhoff F, Kerr J, Helmchen F . Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo. Nat Methods. 2005; 1(1):31-7. DOI: 10.1038/nmeth706. View

Roberts L, Woodford K, Zhou M, Black D, Haggerty J, Tate E . Expression of the thyroid hormone transporters monocarboxylate transporter-8 (SLC16A2) and organic ion transporter-14 (SLCO1C1) at the blood-brain barrier. Endocrinology. 2008; 149(12):6251-61. DOI: 10.1210/en.2008-0378. View

Pizzagalli F, Hagenbuch B, Stieger B, Klenk U, Folkers G, Meier P . Identification of a novel human organic anion transporting polypeptide as a high affinity thyroxine transporter. Mol Endocrinol. 2002; 16(10):2283-96. DOI: 10.1210/me.2001-0309. View

Rossner M, Hirrlinger J, Wichert S, Boehm C, Newrzella D, Hiemisch H . Global transcriptome analysis of genetically identified neurons in the adult cortex. J Neurosci. 2006; 26(39):9956-66. PMC: 6674475. DOI: 10.1523/JNEUROSCI.0468-06.2006. View

Sugiyama D, Kusuhara H, Taniguchi H, Ishikawa S, Nozaki Y, Aburatani H . Functional characterization of rat brain-specific organic anion transporter (Oatp14) at the blood-brain barrier: high affinity transporter for thyroxine. J Biol Chem. 2003; 278(44):43489-95. DOI: 10.1074/jbc.M306933200. View

Garaschuk O . Imaging microcircuit function in healthy and diseased brain. Exp Neurol. 2012; 242:41-9. DOI: 10.1016/j.expneurol.2012.02.009. View

Grijota-Martinez C, Diez D, Morreale de Escobar G, Bernal J, Morte B . Lack of action of exogenously administered T3 on the fetal rat brain despite expression of the monocarboxylate transporter 8. Endocrinology. 2011; 152(4):1713-21. DOI: 10.1210/en.2010-1014. View

Mui J, Willis K, Hao Z, Berkowitz A . Distributions of active spinal cord neurons during swimming and scratching motor patterns. J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2012; 198(12):877-89. DOI: 10.1007/s00359-012-0758-6. View

10.

Heuer H, Visser T . The pathophysiological consequences of thyroid hormone transporter deficiencies: Insights from mouse models. Biochim Biophys Acta. 2012; 1830(7):3974-8. DOI: 10.1016/j.bbagen.2012.04.009. View

11.

Matthias K, Kirchhoff F, Seifert G, Huttmann K, Matyash M, Kettenmann H . Segregated expression of AMPA-type glutamate receptors and glutamate transporters defines distinct astrocyte populations in the mouse hippocampus. J Neurosci. 2003; 23(5):1750-8. PMC: 6741945. View

12.

Chu C, Li J, Boado R, Pardridge W . Blood-brain barrier genomics and cloning of a novel organic anion transporter. J Cereb Blood Flow Metab. 2007; 28(2):291-301. PMC: 2892270. DOI: 10.1038/sj.jcbfm.9600538. View

13.

Pannasch U, Vargova L, Reingruber J, Ezan P, Holcman D, Giaume C . Astroglial networks scale synaptic activity and plasticity. Proc Natl Acad Sci U S A. 2011; 108(20):8467-72. PMC: 3100942. DOI: 10.1073/pnas.1016650108. View

14.

Kang J, Kang N, Yu Y, Zhang J, Petersen N, Tian G . Sulforhodamine 101 induces long-term potentiation of intrinsic excitability and synaptic efficacy in hippocampal CA1 pyramidal neurons. Neuroscience. 2010; 169(4):1601-9. PMC: 2918738. DOI: 10.1016/j.neuroscience.2010.06.020. View

15.

Ehinger B, Zucker C, Bruun A, Adolph A . In vivo staining of oligodendroglia in the rabbit retina. Glia. 1994; 10(1):40-8. DOI: 10.1002/glia.440100106. View

16.

Nimmerjahn A, Helmchen F . In vivo labeling of cortical astrocytes with sulforhodamine 101 (SR101). Cold Spring Harb Protoc. 2012; 2012(3):326-34. DOI: 10.1101/pdb.prot068155. View

17.

Szoke K, Hartel K, Grass D, Hirrlinger P, Hirrlinger J, Hulsmann S . Glycine transporter 1 expression in the ventral respiratory group is restricted to protoplasmic astrocytes. Brain Res. 2006; 1119(1):182-9. DOI: 10.1016/j.brainres.2006.08.089. View

18.

Bronger H, Konig J, Kopplow K, Steiner H, Ahmadi R, Herold-Mende C . ABCC drug efflux pumps and organic anion uptake transporters in human gliomas and the blood-tumor barrier. Cancer Res. 2005; 65(24):11419-28. DOI: 10.1158/0008-5472.CAN-05-1271. View

19.

Ikegaya Y, Le Bon-Jego M, Yuste R . Large-scale imaging of cortical network activity with calcium indicators. Neurosci Res. 2005; 52(2):132-8. DOI: 10.1016/j.neures.2005.02.004. View

20.

Adli M, Zhu J, Bernstein B . Genome-wide chromatin maps derived from limited numbers of hematopoietic progenitors. Nat Methods. 2010; 7(8):615-8. PMC: 2924612. DOI: 10.1038/nmeth.1478. View