The Tyrosine Phosphatase SHP-2 Dephosphorylated by ALV-J Via Its Env Efficiently Promotes ALV-J Replication

Overview

Journal Virulence

Specialty Microbiology

Date 2021 Jun 25

PMID 34167452

Citations 3

Authors

Tuofan Li

Jing Xie

Xiaohui Yao

Jun Zhang

Chunping Li

Dan Ren

Luyuan Li

Quan Xie

Hongxia Shao

Aijian Qin

Jianqiang Ye

Affiliations

Soon will be listed here.

Abstract

Avian leukosis virus subgroup J (ALV-J) generally induces hemangioma, myeloid leukosis, and immunosuppression in chickens, causing significant poultry industry economic losses worldwide. The unusual gene of ALV-J, with low homology to other subgroups of ALVs, is associated with its unique pathogenesis. However, the exact molecular basis for the pathogenesis and oncogenesis of ALV-J is still not fully understood. In this study, ALV-J infection and the overexpression of Env could efficiently downregulate the phosphorylation of SHP-2 (pSHP-2) and . The membrane-spanning domain (MSD) in Env Gp37 was the functional domain responsible for pSHP-2 downregulation. Moreover, the overexpression of SHP-2 could effectively promote the replication of ALV-J, whereas knockout or allosteric inhibition of SHP-2 could inhibit ALV-J replication. In addition, the knockout of endogenous chicken SHP-2 could significantly increase the proliferation ability of DF-1 cells. All these data demonstrate that SHP-2 dephosphorylated by ALV-J Env could efficiently promote ALV-J replication, highlighting the important role of SHP-2 in the pathogenesis of ALV-J and providing a new target for developing antiviral drugs against ALV-J.

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