Pomegranate Peel As a Phytogenic in Broiler Chickens: Influence Upon Antioxidant, Lipogenesis and Hypotensive Response

Overview

Journal Vet Med Sci

Specialty Veterinary Medicine

Date 2021 Jun 16

PMID 34132060

Citations 10

Authors

Behnam Ahmadipour

Sajad Pat

Samira Abaszadeh

Hossein Hassanpour

Fariborz Khajali

Affiliations

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Abstract

The aim of this study was to evaluate antioxidant, antihyperlipidemic and hypotensive properties of pomegranate peel (PP) on antioxidant status, fat deposition, lipid peroxidation and pulmonary hypertensive response in broiler chickens. A total of 375 one-day-old male broilers (Cobb 500) were randomly assigned to five treatments included dietary PP levels of 0, 2.5, 5.0, 7.5 and 10 g/kg. Supplementation of PP at 7.5 and 10 g/kg resulted in significant upregulation of hepatic catalase (p < 0.004) and superoxide dismutase1 (SOD1; p < 0.05), which reflected in decreased concentration of circulatory malondialdehyde (MDA). Dietary inclusion of PP at 7.5 and 1.0 g/kg significantly decreased serum concentrations of triglycerides (p < 0.004) and cholesterol (p < 0.006) with concomitant decrease in abdominal fat deposition (p < 0.05). The antihyperlipidemic effect of PP was mediated through down-regulation of peroxisome proliferator activated receptor alpha (PPARα). Hypotensive effect of PP was also observed at 7.5 and 10 g/kg as reduced heart weight and the right-to-total ventricular weight ratio (RV/TV) and decreased mortality from pulmonary hypertension. The hypotensive property of PP was associated with increased concentration of serum nitric oxide. In conclusion, this study revealed antioxidative, antihyperlipidemic and hypotensive effects of PP at 7.5 and 10 g/kg in broiler chickens exposed to hypobaric hypoxia. Health-beneficial effects of PP suggest this product as a promising multi-functional phytogenic feed additive for broiler chickens.

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References

Shao J, Wang P, Liu A, Du X, Bai J, Chen M . Punicalagin Prevents Hypoxic Pulmonary Hypertension via Anti-Oxidant Effects in Rats. Am J Chin Med. 2016; 44(4):785-801. DOI: 10.1142/S0192415X16500439. View

Wolfe K, Wu X, Liu R . Antioxidant activity of apple peels. J Agric Food Chem. 2003; 51(3):609-14. DOI: 10.1021/jf020782a. View

Cawthon D, McNew R, Beers K, Bottje W . Evidence of mitochondrial dysfunction in broilers with pulmonary hypertension syndrome (Ascites): effect of t-butyl hydroperoxide on hepatic mitochondrial function, glutathione, and related thiols. Poult Sci. 1999; 78(1):114-24. DOI: 10.1093/ps/78.1.114. View

Ahmadipour B, Sharifi M, Khajali F . Pulmonary hypertensive response of broiler chickens to arginine and guanidinoacetic acid under high-altitude hypoxia. Acta Vet Hung. 2018; 66(1):116-124. DOI: 10.1556/004.2018.011. View

Ahmadipour B, Hassanpour H, Khajali F . Evaluation of hepatic lipogenesis and antioxidant status of broiler chickens fed mountain celery. BMC Vet Res. 2018; 14(1):234. PMC: 6088407. DOI: 10.1186/s12917-018-1561-6. View

Arab H, Jamshidi R, Rassouli A, Shams G, Hassanzadeh M . Generation of hydroxyl radicals during ascites experimentally induced in broilers. Br Poult Sci. 2006; 47(2):216-22. DOI: 10.1080/00071660600611102. View

Xu K, Zhu C, Kim M, Yamahara J, Li Y . Pomegranate flower ameliorates fatty liver in an animal model of type 2 diabetes and obesity. J Ethnopharmacol. 2009; 123(2):280-7. DOI: 10.1016/j.jep.2009.03.035. View

Ahmadipour B, Pat S, Abaszadeh S, Hassanpour H, Khajali F . Pomegranate peel as a phytogenic in broiler chickens: Influence upon antioxidant, lipogenesis and hypotensive response. Vet Med Sci. 2021; 7(5):1907-1913. PMC: 8464295. DOI: 10.1002/vms3.556. View

Derakhshan Z, Ferrante M, Tadi M, Ansari F, Heydari A, Hosseini M . Antioxidant activity and total phenolic content of ethanolic extract of pomegranate peels, juice and seeds. Food Chem Toxicol. 2018; 114:108-111. DOI: 10.1016/j.fct.2018.02.023. View

10.

Zuo L, Rose B, Roberts W, He F, Banes-Berceli A . Molecular characterization of reactive oxygen species in systemic and pulmonary hypertension. Am J Hypertens. 2014; 27(5):643-50. DOI: 10.1093/ajh/hpt292. View

11.

Esmaillzadeh A, Tahbaz F, Gaieni I, Alavi-Majd H, Azadbakht L . Cholesterol-lowering effect of concentrated pomegranate juice consumption in type II diabetic patients with hyperlipidemia. Int J Vitam Nutr Res. 2006; 76(3):147-51. DOI: 10.1024/0300-9831.76.3.147. View

12.

Hassanpour H, Bahadoran S, Farhadfar F, Chamali Z, Nazari H, Kaewduangta W . Identification of reliable reference genes for quantitative real-time PCR in lung and heart of pulmonary hypertensive chickens. Poult Sci. 2018; 97(11):4048-4056. DOI: 10.3382/ps/pey258. View

13.

Salgado J, Baroni Ferreira T, de Oliveira Biazotto F, Dias C . Increased antioxidant content in juice enriched with dried extract of pomegranate (Punica granatum) peel. Plant Foods Hum Nutr. 2012; 67(1):39-43. DOI: 10.1007/s11130-011-0264-y. View

14.

Tickle P, Hutchinson J, Codd J . Energy allocation and behaviour in the growing broiler chicken. Sci Rep. 2018; 8(1):4562. PMC: 5852157. DOI: 10.1038/s41598-018-22604-2. View

15.

Khajali F, Khajali Z . L-carnitine supplementation decreases lipid peroxidation and improves cardiopulmonary function in broiler chickens reared at high altitude. Acta Vet Hung. 2014; 62(4):489-99. DOI: 10.1556/AVet.2014.022. View

16.

Cui H, Zheng M, Liu R, Zhao G, Chen J, Wen J . Liver dominant expression of fatty acid synthase (FAS) gene in two chicken breeds during intramuscular-fat development. Mol Biol Rep. 2011; 39(4):3479-84. DOI: 10.1007/s11033-011-1120-8. View

17.

Gessner D, Ringseis R, Eder K . Potential of plant polyphenols to combat oxidative stress and inflammatory processes in farm animals. J Anim Physiol Anim Nutr (Berl). 2016; 101(4):605-628. DOI: 10.1111/jpn.12579. View

18.

Frisard M, Ravussin E . Energy metabolism and oxidative stress: impact on the metabolic syndrome and the aging process. Endocrine. 2006; 29(1):27-32. DOI: 10.1385/ENDO:29:1:27. View

19.

Fruchart J . Peroxisome proliferator-activated receptor-alpha (PPARalpha): at the crossroads of obesity, diabetes and cardiovascular disease. Atherosclerosis. 2009; 205(1):1-8. DOI: 10.1016/j.atherosclerosis.2009.03.008. View

20.

Surai P, Kochish I, Fisinin V, Kidd M . Antioxidant Defence Systems and Oxidative Stress in Poultry Biology: An Update. Antioxidants (Basel). 2019; 8(7). PMC: 6680731. DOI: 10.3390/antiox8070235. View