LncRNA SNHG7 Promotes Cell Proliferation in Glioma by Acting As a Competing Endogenous RNA and Sponging MiR-138-5p to Regulate EZH2 Expression

Overview

Journal Oncol Lett

Specialty Oncology

Date 2021 Jun 11

PMID 34113393

Citations 4

Authors

Yanyao Deng

Liuyang Cheng

Zhicheng Lv

Hongwei Zhu

Xiangrui Meng

Affiliations

Soon will be listed here.

Abstract

Glioma is the most common type of primary brain cancer in adults. Accumulating studies have reported that long non-coding RNAs (lncRNAs) serve a significant role in the initiation and development of glioma. lncRNA small nucleolar RNA host gene 7 (SNHG7) has been previously demonstrated to serve a role in numerous glioma biological processes, including cell proliferation, invasion and migration. The present study aimed to investigate the role of SNHG7 in glioma through reverse transcription-quantitative PCR, western blotting and cell function assays. The results revealed that SNHG7 expression was upregulated in glioma tissues and cell lines, while microRNA (miR)-138-5p expression was downregulated. Moreover, the knockdown of SNHG7 expression decreased the proliferation of glioma cells. Mechanistic studies demonstrated that SNHG7 downregulated miR-138-5p expression, which subsequently affected the expression levels of its target gene, enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2). In conclusion, the results of the present study suggested that SNHG7 may act as a competing endogenous RNA to sponge miR-138-5p and modulate EZH2 expression. Thus, SNHG7 may enhance glioma proliferation via modulating the miR-138-5p/EZH2 signaling axis.

Citing Articles

Correlation of SNHG7 and BGL3 expression in patients with de novo acute myeloid leukemia; novel insights into lncRNA effect in PI3K signaling context in AML pathogenesis.

Hassani S, Rostami P, Pourtavakol M, Karamashtiani A, Sayyadi M Biochem Biophys Rep. 2024; 40:101850.

PMID: 39469045 PMC: 11513491. DOI: 10.1016/j.bbrep.2024.101850.

Circular RNA- and microRNA-Mediated Post-Transcriptional Regulation of Preadipocyte Differentiation in Adipogenesis: From Expression Profiling to Signaling Pathway.

Huang C, Choo K Int J Mol Sci. 2023; 24(5).

PMID: 36901978 PMC: 10002489. DOI: 10.3390/ijms24054549.

miR-138-5p Inhibits the Growth and Invasion of Glioma Cells by Regulating WEE1.

Gong J, Tang Z, Yu Z, Deng Z, Liu Y, Ren N Anal Cell Pathol (Amst). 2022; 2022:7809882.

PMID: 35127343 PMC: 8816588. DOI: 10.1155/2022/7809882.

Oncogenic Roles of Small Nucleolar RNA Host Gene 7 (SNHG7) Long Noncoding RNA in Human Cancers and Potentials.

Najafi S, Ghafouri-Fard S, Hussen B, Jamal H, Taheri M, Hallajnejad M Front Cell Dev Biol. 2022; 9:809345.

PMID: 35111760 PMC: 8801878. DOI: 10.3389/fcell.2021.809345.

References

Carthew R, Sontheimer E . Origins and Mechanisms of miRNAs and siRNAs. Cell. 2009; 136(4):642-55. PMC: 2675692. DOI: 10.1016/j.cell.2009.01.035. View

Ulitsky I, Bartel D . lincRNAs: genomics, evolution, and mechanisms. Cell. 2013; 154(1):26-46. PMC: 3924787. DOI: 10.1016/j.cell.2013.06.020. View

Cheng D, Fan J, Ma Y, Zhou Y, Qin K, Shi M . LncRNA SNHG7 promotes pancreatic cancer proliferation through ID4 by sponging miR-342-3p. Cell Biosci. 2019; 9:28. PMC: 6431029. DOI: 10.1186/s13578-019-0290-2. View

Wesseling P, Capper D . WHO 2016 Classification of gliomas. Neuropathol Appl Neurobiol. 2017; 44(2):139-150. DOI: 10.1111/nan.12432. View

Wu L, Zhu X, Song Z, Chen D, Guo M, Liang J . Long Non-Coding RNA HOXA-AS2 Enhances The Malignant Biological Behaviors In Glioma By Epigenetically Regulating RND3 Expression. Onco Targets Ther. 2019; 12:9407-9419. PMC: 6844264. DOI: 10.2147/OTT.S225678. View

Gan L, Yang Y, Li Q, Feng Y, Liu T, Guo W . Epigenetic regulation of cancer progression by EZH2: from biological insights to therapeutic potential. Biomark Res. 2018; 6:10. PMC: 5845366. DOI: 10.1186/s40364-018-0122-2. View

Zhou Y, Tian B, Tang J, Wu J, Wang H, Wu Z . SNHG7: A novel vital oncogenic lncRNA in human cancers. Biomed Pharmacother. 2020; 124:109921. DOI: 10.1016/j.biopha.2020.109921. View

Komori T . The 2016 WHO Classification of Tumours of the Central Nervous System: The Major Points of Revision. Neurol Med Chir (Tokyo). 2017; 57(7):301-311. PMC: 5566703. DOI: 10.2176/nmc.ra.2017-0010. View

Xu M, Chen X, Lin K, Zeng K, Liu X, Xu X . lncRNA SNHG6 regulates EZH2 expression by sponging miR-26a/b and miR-214 in colorectal cancer. J Hematol Oncol. 2019; 12(1):3. PMC: 6327409. DOI: 10.1186/s13045-018-0690-5. View

10.

Liu Y, Sun J, Yu J, Ge W, Xiao X, Dai S . LncRNA CACS15 accelerates the malignant progression of ovarian cancer through stimulating EZH2-induced inhibition of APC. Am J Transl Res. 2019; 11(10):6561-6568. PMC: 6834498. View

11.

Ou L, Wang D, Zhang H, Yu Q, Hua F . Decreased Expression of miR-138-5p by lncRNA H19 in Cervical Cancer Promotes Tumor Proliferation. Oncol Res. 2017; 26(3):401-410. PMC: 7844697. DOI: 10.3727/096504017X15017209042610. View

12.

Weller M, Wick W, Aldape K, Brada M, Berger M, Pfister S . Glioma. Nat Rev Dis Primers. 2016; 1:15017. DOI: 10.1038/nrdp.2015.17. View

13.

Zhao L, Sun H, Kong H, Chen Z, Chen B, Zhou M . The Lncrna-TUG1/EZH2 Axis Promotes Pancreatic Cancer Cell Proliferation, Migration and EMT Phenotype Formation Through Sponging Mir-382. Cell Physiol Biochem. 2017; 42(6):2145-2158. DOI: 10.1159/000479990. View

14.

Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R . Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 2004; 36(1):40-5. DOI: 10.1038/ng1285. View

15.

Zhang K, Sun X, Zhou X, Han L, Chen L, Shi Z . Long non-coding RNA HOTAIR promotes glioblastoma cell cycle progression in an EZH2 dependent manner. Oncotarget. 2014; 6(1):537-46. PMC: 4381613. DOI: 10.18632/oncotarget.2681. View

16.

Oberheim Bush N, Chang S, Berger M . Current and future strategies for treatment of glioma. Neurosurg Rev. 2016; 40(1):1-14. DOI: 10.1007/s10143-016-0709-8. View

17.

Davis M . Epidemiology and Overview of Gliomas. Semin Oncol Nurs. 2018; 34(5):420-429. DOI: 10.1016/j.soncn.2018.10.001. View

18.

Pop S, Enciu A, Necula L, Tanase C . Long non-coding RNAs in brain tumours: Focus on recent epigenetic findings in glioma. J Cell Mol Med. 2018; 22(10):4597-4610. PMC: 6156469. DOI: 10.1111/jcmm.13781. View

19.

Yao X, Liu C, Liu C, Xi W, Sun S, Gao Z . lncRNA SNHG7 sponges miR-425 to promote proliferation, migration, and invasion of hepatic carcinoma cells via Wnt/β-catenin/EMT signalling pathway. Cell Biochem Funct. 2019; 37(7):525-533. PMC: 6851833. DOI: 10.1002/cbf.3429. View

20.

Beermann J, Piccoli M, Viereck J, Thum T . Non-coding RNAs in Development and Disease: Background, Mechanisms, and Therapeutic Approaches. Physiol Rev. 2016; 96(4):1297-325. DOI: 10.1152/physrev.00041.2015. View