Advanced Retinal Imaging and Ocular Parameters of the Rhesus Macaque Eye

Overview

Journal Transl Vis Sci Technol

Specialties Biomedical Engineering
Ophthalmology

Date 2021 Jun 10

PMID 34111251

Citations 14

Authors

Kira H Lin

Tu Tran

Soohyun Kim

Sangwan Park

J Timothy Stout

Rui Chen

Jeffrey Rogers

Glenn Yiu

Sara Thomasy

Ala Moshiri

Affiliations

Soon will be listed here.

Abstract

Purpose: To determine the range of normal ocular biometry and perform advanced retinal imaging and functional assessment of the rhesus macaque eye.

Methods: We performed ocular phenotyping on rhesus macaques at the California National Primate Research Center. This process consisted of anterior and posterior segment eye examination by ophthalmologists, advanced retinal imaging, and functional retinal electrophysiology.

Results: Full eye examinations were performed on 142 animals, consisting of pupillary light reflex, tonometry, external examination and photography, anterior slit lamp examination, and posterior segment examination by indirect ophthalmoscopy. Ages of the rhesus macaques ranged from 0.7 to 29 years (mean, 16.4 ± 7.5 years). Anterior segment measurements such as intraocular pressure (n = 142), corneal thickness (n = 84), lens thickness (n = 114), and axial length (n = 114) were acquired. Advanced retinal imaging in the form of fundus photography (n = 78), optical coherence tomography (n = 60), and quantitative autofluorescence (n = 44) was obtained. Electroretinography (n = 75) was used to assay retinal function. Quantitative analyses of the macular structure, retinal layer segmentation, and rod and cone photoreceptor electrical responses are reported. Quantitative assessments were made and variations between sexes were analyzed to compare with established sex changes in human eyes.

Conclusions: The rhesus macaque has an ocular structure and function very similar to that of the human eye. In particular macular structure and retinal function is very similar to humans, making this species particularly useful for the study of macular biology and development of therapies for cone photoreceptor disorders.

Translational Relevance: Rhesus macaques are an ideal model for future vision science studies of human eye diseases.

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References

Gliem M, Muller P, Finger R, McGuinness M, Holz F, Charbel Issa P . Quantitative Fundus Autofluorescence in Early and Intermediate Age-Related Macular Degeneration. JAMA Ophthalmol. 2016; 134(7):817-24. DOI: 10.1001/jamaophthalmol.2016.1475. View

Bhardwaj V, Rajeshbhai G . Axial length, anterior chamber depth-a study in different age groups and refractive errors. J Clin Diagn Res. 2013; 7(10):2211-2. PMC: 3843406. DOI: 10.7860/JCDR/2013/7015.3473. View

Dubbelman M, van der Heijde G, Weeber H . The thickness of the aging human lens obtained from corrected Scheimpflug images. Optom Vis Sci. 2001; 78(6):411-6. DOI: 10.1097/00006324-200106000-00013. View

Lei B, Bush R, Milam A, Sieving P . Human melanoma-associated retinopathy (MAR) antibodies alter the retinal ON-response of the monkey ERG in vivo. Invest Ophthalmol Vis Sci. 2000; 41(1):262-6. View

Jonas R, Wang Y, Yang H, Li J, Xu L, Panda-Jonas S . Optic Disc-Fovea Distance, Axial Length and Parapapillary Zones. The Beijing Eye Study 2011. PLoS One. 2015; 10(9):e0138701. PMC: 4577126. DOI: 10.1371/journal.pone.0138701. View

Liu H, Ji X, Dhaliwal S, Rahman S, McFarlane M, Tumber A . Evaluation of light- and dark-adapted ERGs using a mydriasis-free, portable system: clinical classifications and normative data. Doc Ophthalmol. 2018; 137(3):169-181. DOI: 10.1007/s10633-018-9660-z. View

Ribka E, Dubielzig R . Multiple ophthalmic abnormalities in an infant rhesus macaque (Macaca mulatta). J Med Primatol. 2008; 37 Suppl 1:16-9. PMC: 2658013. DOI: 10.1111/j.1600-0684.2007.00263.x. View

Wakabayashi K, Gieser J, Sieving P . Aspartate separation of the scotopic threshold response (STR) from the photoreceptor a-wave of the cat and monkey ERG. Invest Ophthalmol Vis Sci. 1988; 29(11):1615-22. View

Colman R, Anderson R, Johnson S, Kastman E, Kosmatka K, Beasley T . Caloric restriction delays disease onset and mortality in rhesus monkeys. Science. 2009; 325(5937):201-4. PMC: 2812811. DOI: 10.1126/science.1173635. View

10.

Sparrow J, Duncker T . Fundus Autofluorescence and RPE Lipofuscin in Age-Related Macular Degeneration. J Clin Med. 2015; 3(4):1302-21. PMC: 4358814. DOI: 10.3390/jcm3041302. View

11.

Wang Y, Tran T, Firl K, Huang N, Yasin O, van Kuijk F . Quantitative fundus autofluorescence in smokers compared to non-smokers. Exp Eye Res. 2019; 184:48-55. DOI: 10.1016/j.exer.2019.04.004. View

12.

Rasmussen C, Kaufman P . Primate glaucoma models. J Glaucoma. 2005; 14(4):311-4. DOI: 10.1097/01.ijg.0000169409.01635.bc. View

13.

Moshiri A, Chen R, Kim S, Harris R, Li Y, Raveendran M . A nonhuman primate model of inherited retinal disease. J Clin Invest. 2019; 129(2):863-874. PMC: 6355306. DOI: 10.1172/JCI123980. View

14.

Delori F, Greenberg J, Woods R, Fischer J, Duncker T, Sparrow J . Quantitative measurements of autofluorescence with the scanning laser ophthalmoscope. Invest Ophthalmol Vis Sci. 2011; 52(13):9379-90. PMC: 3250263. DOI: 10.1167/iovs.11-8319. View

15.

Regal S, OConnor D, Brige P, Delattre R, Djenizian T, Ramuz M . Determination of optical parameters of the porcine eye and development of a simulated model. J Biophotonics. 2019; 12(11):e201800398. DOI: 10.1002/jbio.201800398. View

16.

Bush R, Sieving P . Inner retinal contributions to the primate photopic fast flicker electroretinogram. J Opt Soc Am A Opt Image Sci Vis. 1996; 13(3):557-65. DOI: 10.1364/josaa.13.000557. View

17.

Fischer M, Zobor D, Keliris G, Shao Y, Seeliger M, Haverkamp S . Detailed functional and structural characterization of a macular lesion in a rhesus macaque. Doc Ophthalmol. 2012; 125(3):179-94. PMC: 7548104. DOI: 10.1007/s10633-012-9340-3. View

18.

Yang H, Reynaud J, Lockwood H, Williams G, Hardin C, Reyes L . The connective tissue phenotype of glaucomatous cupping in the monkey eye - Clinical and research implications. Prog Retin Eye Res. 2017; 59:1-52. PMC: 5603293. DOI: 10.1016/j.preteyeres.2017.03.001. View

19.

Quigley H, Brown A, Morrison J, Drance S . The size and shape of the optic disc in normal human eyes. Arch Ophthalmol. 1990; 108(1):51-7. DOI: 10.1001/archopht.1990.01070030057028. View

20.

Freeberg F, NIXON G, Reer P, Weaver J, Bruce R, Griffith J . Human and rabbit eye responses to chemical insult. Fundam Appl Toxicol. 1986; 7(4):626-34. DOI: 10.1016/0272-0590(86)90112-0. View