Design, Methodology, and Preliminary Results of the Non-human Primates Eye Study

Overview

Journal BMC Ophthalmol

Publisher Biomed Central

Specialty Ophthalmology

Date 2023 Feb 8

PMID 36750922

Authors

Jian Wu

Wei Liu

Sirui Zhu

Hongyi Liu

Kezhe Chen

Yingting Zhu

Zhidong Li

Chenlong Yang

Lijie Pan

Ruyue Li

Caixia Lin

Jiaxin Tian

Jiaoyan Ren

Liangzhi Xu

Hanxiang Yu

Fagao Luo

Zhiwei Huang

Wenru Su

Ningli Wang

Yehong Zhuo

Affiliations

Soon will be listed here.

Abstract

Purpose: To describe the normative profile of ophthalmic parameters in a healthy cynomolgus monkey colony, and to identify the characteristic of the spontaneous ocular disease non-human primates (NHP) models.

Methods: The NHP eye study was a cross-sectional on-site ocular examination with about 1,000 macaques held in Guangdong Province, southeastern China. The NHPs (Macaca fascicularis, cynomolgus) in this study included middle-aged individuals with a high prevalence of the ocular disease. The NHP eye study (NHPES) performed the information including systematic data and ocular data. Ocular examination included measurement of intraocular pressure (IOP), anterior segment- optical coherence tomography (OCT), slit-lamp examination, fundus photography, autorefraction, electroretinography, etc. Ocular diseases included measurement of refractive error, anisometropia, cataract, pterygium, etc. RESULTS: A total of 1148 subjects were included and completed the ocular examination. The average age was 16.4 ± 4.93 years. Compared to the male participants, the females in the NHPES had shorter axial length and the mean Average retinal nerve fiber layer (RNFL) thickness (except for the nasal quadrants). The mean IOP, anterior chamber depth, lens thickness, axial length, central corneal thickness, choroid thickness and other parameters were similar in each group.

Conclusion: The NHPES is a unique and high-quality study, this is the first large macaque monkey cohort study focusing on ocular assessment along with comprehensive evaluation. Results from the NHPES will provide important information about the normal range of ophthalmic measurements in NHP.

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References

Delcourt C, Le Goff M, von Hanno T, Mirshahi A, Khawaja A, Verhoeven V . The Decreasing Prevalence of Nonrefractive Visual Impairment in Older Europeans: A Meta-analysis of Published and Unpublished Data. Ophthalmology. 2018; 125(8):1149-1159. DOI: 10.1016/j.ophtha.2018.02.005. View

Liang Y, Friedman D, Zhou Q, Yang X, Sun L, Guo L . Prevalence of primary open angle glaucoma in a rural adult Chinese population: the Handan eye study. Invest Ophthalmol Vis Sci. 2011; 52(11):8250-7. DOI: 10.1167/iovs.11-7472. View

Jonas J, Aung T, Bourne R, Bron A, Ritch R, Panda-Jonas S . Glaucoma. Lancet. 2017; 390(10108):2183-2193. DOI: 10.1016/S0140-6736(17)31469-1. View

Pasquale L, Gong L, Wiggs J, Pan L, Yang Z, Wu M . Development of Primary Open Angle Glaucoma-Like Features in a Rhesus Macaque Colony From Southern China. Transl Vis Sci Technol. 2021; 10(9):20. PMC: 8374995. DOI: 10.1167/tvst.10.9.20. View

Zangalli C, Reis A, Vianna J, Vasconcellos J, Costa V . Interocular Asymmetry of Minimum Rim Width and Retinal Nerve Fiber Layer Thickness in Healthy Brazilian Individuals. J Glaucoma. 2018; 27(12):1136-1141. DOI: 10.1097/IJG.0000000000001078. View

Lee C, Afshari N . The global state of cataract blindness. Curr Opin Ophthalmol. 2016; 28(1):98-103. DOI: 10.1097/ICU.0000000000000340. View

Appukuttan B, Giridhar A, Gopalakrishnan M, Sivaprasad S . Normative spectral domain optical coherence tomography data on macular and retinal nerve fiber layer thickness in Indians. Indian J Ophthalmol. 2013; 62(3):316-21. PMC: 4061670. DOI: 10.4103/0301-4738.116466. View

Li J, Welchowski T, Schmid M, Letow J, Wolpers C, Pascual-Camps I . Prevalence, incidence and future projection of diabetic eye disease in Europe: a systematic review and meta-analysis. Eur J Epidemiol. 2019; 35(1):11-23. DOI: 10.1007/s10654-019-00560-z. View

Klein R, Klein B, Linton K . Prevalence of age-related maculopathy. The Beaver Dam Eye Study. Ophthalmology. 1992; 99(6):933-43. DOI: 10.1016/s0161-6420(92)31871-8. View

10.

Dwelle J, Liu S, Wang B, McElroy A, Ho D, Markey M . Thickness, phase retardation, birefringence, and reflectance of the retinal nerve fiber layer in normal and glaucomatous non-human primates. Invest Ophthalmol Vis Sci. 2012; 53(8):4380-95. PMC: 3394663. DOI: 10.1167/iovs.11-9130. View

11.

Picaud S, Dalkara D, Marazova K, Goureau O, Roska B, Sahel J . The primate model for understanding and restoring vision. Proc Natl Acad Sci U S A. 2019; 116(52):26280-26287. PMC: 6936588. DOI: 10.1073/pnas.1902292116. View

12.

Zhang Y, Toris C, Liu Y, Ye W, Gong H . Morphological and hydrodynamic correlates in monkey eyes with laser induced glaucoma. Exp Eye Res. 2009; 89(5):748-56. DOI: 10.1016/j.exer.2009.06.015. View

13.

Troilo D, Smith 3rd E, Nickla D, Ashby R, Tkatchenko A, Ostrin L . IMI - Report on Experimental Models of Emmetropization and Myopia. Invest Ophthalmol Vis Sci. 2019; 60(3):M31-M88. PMC: 6738517. DOI: 10.1167/iovs.18-25967. View

14.

Ollivier F, Brooks D, Komaromy A, Kallberg M, Andrew S, Sapp H . Corneal thickness and endothelial cell density measured by non-contact specular microscopy and pachymetry in Rhesus macaques (Macaca mulatta) with laser-induced ocular hypertension. Exp Eye Res. 2003; 76(6):671-7. DOI: 10.1016/s0014-4835(03)00055-1. View

15.

Duan X, Liang Y, Friedman D, Sun L, Wong T, Tao Q . Normal macular thickness measurements using optical coherence tomography in healthy eyes of adult Chinese persons: the Handan Eye Study. Ophthalmology. 2010; 117(8):1585-94. DOI: 10.1016/j.ophtha.2009.12.036. View

16.

. Grading diabetic retinopathy from stereoscopic color fundus photographs--an extension of the modified Airlie House classification. ETDRS report number 10. Early Treatment Diabetic Retinopathy Study Research Group. Ophthalmology. 1991; 98(5 Suppl):786-806. View

17.

Jonas J, Cheung C, Panda-Jonas S . Updates on the Epidemiology of Age-Related Macular Degeneration. Asia Pac J Ophthalmol (Phila). 2017; 6(6):493-497. DOI: 10.22608/APO.2017251. View

18.

Burton M, Ramke J, Marques A, Bourne R, Congdon N, Jones I . The Lancet Global Health Commission on Global Eye Health: vision beyond 2020. Lancet Glob Health. 2021; 9(4):e489-e551. PMC: 7966694. DOI: 10.1016/S2214-109X(20)30488-5. View

19.

Alasil T, Wang K, Keane P, Lee H, Baniasadi N, de Boer J . Analysis of normal retinal nerve fiber layer thickness by age, sex, and race using spectral domain optical coherence tomography. J Glaucoma. 2012; 22(7):532-41. DOI: 10.1097/IJG.0b013e318255bb4a. View

20.

Yan G, Zhang G, Fang X, Zhang Y, Li C, Ling F . Genome sequencing and comparison of two nonhuman primate animal models, the cynomolgus and Chinese rhesus macaques. Nat Biotechnol. 2011; 29(11):1019-23. DOI: 10.1038/nbt.1992. View