Novel Lamprey Antibody Recognizes Terminal Sulfated Galactose Epitopes on Mammalian Glycoproteins

Overview

Journal Commun Biol

Specialty Biology

Date 2021 Jun 4

PMID 34083726

Citations 11

Authors

Tanya R McKitrick

Steffen M Bernard

Alexander J Noll

Bernard C Collins

Christoffer K Goth

Alyssa M McQuillan

Jamie Heimburg-Molinaro

Brantley R Herrin

Ian A Wilson

Max D Cooper

Richard D Cummings

Affiliations

Soon will be listed here.

Abstract

The terminal galactose residues of N- and O-glycans in animal glycoproteins are often sialylated and/or fucosylated, but sulfation, such as 3-O-sulfated galactose (3-O-SGal), represents an additional, but poorly understood modification. To this end, we have developed a novel sea lamprey variable lymphocyte receptor (VLR) termed O6 to explore 3-O-SGal expression. O6 was engineered as a recombinant murine IgG chimera and its specificity and affinity to the 3-O-SGal epitope was defined using a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays. 3-O-SGal is expressed on N-glycans of many plasma and tissue glycoproteins, but recognition by O6 is often masked by sialic acid and thus exposed by treatment with neuraminidase. O6 recognizes many human tissues, consistent with expression of the cognate sulfotransferases (GAL3ST-2 and GAL3ST-3). The availability of O6 for exploring 3-O-SGal expression could lead to new biomarkers for disease and aid in understanding the functional roles of terminal modifications of glycans and relationships between terminal sulfation, sialylation and fucosylation.

Citing Articles

Thyroid Carcinoma Glycoproteins Express Altered -Glycans with 3-O-Sulfated Galactose Residues.

Broekhuis J, Lu D, Aryal R, Matsumoto Y, Pepi L, Chaves N Biomolecules. 2025; 14(12.

PMID: 39766189 PMC: 11727208. DOI: 10.3390/biom14121482.

High throughput long-read sequencing of circulating lymphocytes of the evolutionarily distant sea lamprey reveals diversity and common elements of the variable lymphocyte receptor B (VLRB) repertoire.

Zia A, Orozco A, Fang I, Tang A, Mendoza Viruega A, Dong S Front Immunol. 2024; 15:1427075.

PMID: 39170622 PMC: 11335541. DOI: 10.3389/fimmu.2024.1427075.

Reusable glycan microarrays using a microwave assisted wet-erase (MAWE) process.

Mehta A, Tilton C, Muerner L, von Gunten S, Heimburg-Molinaro J, Cummings R Glycobiology. 2023; 34(2).

PMID: 37962922 PMC: 10969520. DOI: 10.1093/glycob/cwad091.

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Engineered Glycan-Binding Proteins for Recognition of the Thomsen-Friedenreich Antigen and Structurally Related Disaccharides.

Ward E, Zamora C, Schocker N, Ghosh S, Kizer M, Imperiali B ACS Chem Biol. 2022; 18(1):70-80.

PMID: 36525666 PMC: 9868099. DOI: 10.1021/acschembio.2c00683.

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