Experimental Platform to Study Spiking Pattern Propagation in Modular Networks In Vitro

Overview

Journal Brain Sci

Publisher MDPI

Date 2021 Jun 2

PMID 34071257

Citations 5

Authors

Yana Pigareva

Arseniy Gladkov

Vladimir Kolpakov

Irina Mukhina

Anton Bukatin

Victor B Kazantsev

Alexey Pimashkin

Affiliations

Soon will be listed here.

Abstract

The structured organization of connectivity in neural networks is associated with highly efficient information propagation and processing in the brain, in contrast with disordered homogeneous network architectures. Using microfluidic methods, we engineered modular networks of cultures using dissociated cells with unidirectional synaptic connections formed by asymmetric microchannels. The complexity of the microchannel geometry defined the strength of the synaptic connectivity and the properties of spiking activity propagation. In this study, we developed an experimental platform to study the effects of synaptic plasticity on a network level with predefined locations of unidirectionally connected cellular assemblies using multisite extracellular electrophysiology.

Citing Articles

Microfluidic Chip for Studying the Mechanisms of Allogeneic Progenitor Neuronal Cells Integration into a Mature Neural Network In Vitro.

Gladkov A, Zemlyanskov M, Pigareva Y, Kolpakov V, Kazantsev V, Mukhina I Bull Exp Biol Med. 2025; 178(3):387-392.

PMID: 39951228 DOI: 10.1007/s10517-025-06342-w.

The Profile of Network Spontaneous Activity and Functional Organization Interplay in Hierarchically Connected Modular Neural Networks In Vitro.

Pigareva Y, Gladkov A, Kolpakov V, Kazantsev V, Mukhina I, Pimashkin A Micromachines (Basel). 2024; 15(6).

PMID: 38930702 PMC: 11205292. DOI: 10.3390/mi15060732.

Living-Neuron-Based Autogenerator.

Gerasimova S, Beltyukova A, Fedulina A, Matveeva M, Lebedeva A, Pisarchik A Sensors (Basel). 2023; 23(16).

PMID: 37631552 PMC: 10458024. DOI: 10.3390/s23167016.

Portrait of intense communications within microfluidic neural networks.

Dupuit V, Briancon-Marjollet A, Delacour C Sci Rep. 2023; 13(1):12306.

PMID: 37516789 PMC: 10387102. DOI: 10.1038/s41598-023-39477-9.

Microfluidic Bi-Layer Platform to Study Functional Interaction between Co-Cultured Neural Networks with Unidirectional Synaptic Connectivity.

Pigareva Y, Gladkov A, Kolpakov V, Bukatin A, Li S, Kazantsev V Micromachines (Basel). 2023; 14(4).

PMID: 37421068 PMC: 10146337. DOI: 10.3390/mi14040835.

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