Isolation, Physicochemical Properties, and Structural Characteristics of Arabinoxylan from Hull-Less Barley

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2021 Jun 2

PMID 34069493

Citations 1

Authors

Haoyingye Yao

Yuxiao Wang

Junyi Yin

Shaoping Nie

Mingyong Xie

Affiliations

Soon will be listed here.

Abstract

Arabinoxylan (HBAX-60) was fractioned from alkaline-extracted arabinoxylan (HBAX) in the whole grain of hull-less barley ( L. Hook. f. ) by 60% ethanol precipitation, which was studied for physicochemical properties and structure elucidation. Highly purified HBAX-60 mainly composed of arabinose (40.7%) and xylose (59.3%) was created. The methylation and NMR analysis of HBAX-60 indicated that a low-branched β-(1→4)-linked xylan backbone possessed un-substituted (1,4-linked β-Xyl, 36.2%), mono-substituted (β-1,3,4-linked Xyl, 5.9%), and di-substituted (1,2,3,4-linked β-Xyl, 12.1%) xylose units as the main chains, though other residues (α-Ara-(1→, β-Xyl-(1→, α-Ara-(1→3)-α-Ara-(1→ or β-Xyl-(1→3)-α-Ara-(1→) were also determined. Additionally, HBAX-60 exhibited random coil conformation in a 0.1 M NaNO solution. This work provides the properties and structural basis of the hull-less barley-derived arabinoxylan, which facilitates further research for exploring the structure-function relationship and application of arabinoxylan from hull-less barley.

Citing Articles

Arabinoxylan as well as β-glucan in barley promotes GLP-1 secretion by increasing short-chain fatty acids production.

Mio K, Ogawa R, Tadenuma N, Aoe S Biochem Biophys Rep. 2022; 32:101343.

PMID: 36123993 PMC: 9482107. DOI: 10.1016/j.bbrep.2022.101343.

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