The Analysis of Phenolic Compounds in Walnut Husk and Pellicle by UPLC-Q-Orbitrap HRMS and HPLC

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2021 Jun 2

PMID 34069333

Citations 9

Authors

Fang Sheng

Bangyan Hu

Qiang Jin

Jiangbo Wang

Cuiyun Wu

Zhengrong Luo

Affiliations

Soon will be listed here.

Abstract

Husk and pellicle as the agri-food waste in the walnut-product industry are in soaring demand because of their rich polyphenol content. This study investigated the differential compounds related to walnut polyphenol between husk and pellicle during fruit development stage. By using ultra-high performance liquid chromatography-quadrupole-orbitrap (UHPLC-Q-Orbitrap), a total of 110 bioactive components, including hydrolysable tannins, flavonoids, phenolic acids and quinones, were tentatively identified, 33 of which were different between husk and pellicle. The trend of dynamic content of 16 polyphenols was clarified during walnut development stage by high-performance liquid chromatography (HPLC). This is the first time to comprehensive identification of phenolic compounds in walnut husk and pellicle, and our results indicated that the pellicle is a rich resource of polyphenols. The dynamic trend of some polyphenols was consistent with total phenols. The comprehensive characterization of walnut polyphenol and quantification of main phenolic compounds will be beneficial for understanding the potential application value of walnut and for exploiting its metabolism pathway.

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References

Persic M, Mikulic-Petkovsek M, Slatnar A, Solar A, Veberic R . Changes in phenolic profiles of red-colored pellicle walnut and hazelnut kernel during ripening. Food Chem. 2018; 252:349-355. DOI: 10.1016/j.foodchem.2018.01.124. View

Gomez-Caravaca A, Verardo V, Segura-Carretero A, Caboni M, Fernandez-Gutierrez A . Development of a rapid method to determine phenolic and other polar compounds in walnut by capillary electrophoresis-electrospray ionization time-of-flight mass spectrometry. J Chromatogr A. 2008; 1209(1-2):238-45. DOI: 10.1016/j.chroma.2008.08.117. View

Hardman W . Walnuts have potential for cancer prevention and treatment in mice. J Nutr. 2014; 144(4 Suppl):555S-560S. PMC: 3952627. DOI: 10.3945/jn.113.188466. View

Jahanban-Esfahlan A, Ostadrahimi A, Tabibiazar M, Amarowicz R . A Comprehensive Review on the Chemical Constituents and Functional Uses of Walnut ( spp.) Husk. Int J Mol Sci. 2019; 20(16). PMC: 6719079. DOI: 10.3390/ijms20163920. View

Koleckar V, Kubikova K, Rehakova Z, Kuca K, Jun D, Jahodar L . Condensed and hydrolysable tannins as antioxidants influencing the health. Mini Rev Med Chem. 2008; 8(5):436-47. DOI: 10.2174/138955708784223486. View

Colaric M, Veberic R, Solar A, Hudina M, Stampar F . Phenolic acids, syringaldehyde, and juglone in fruits of different cultivars of Juglans regia L. J Agric Food Chem. 2005; 53(16):6390-6. DOI: 10.1021/jf050721n. View

Vasic V, Gasic U, Stankovic D, Lusic D, Vukic-Lusic D, Milojkovic-Opsenica D . Towards better quality criteria of European honeydew honey: Phenolic profile and antioxidant capacity. Food Chem. 2018; 274:629-641. DOI: 10.1016/j.foodchem.2018.09.045. View

Li L, Song L, Sun X, Yan S, Huang W, Liu P . Characterisation of phenolics in fruit septum of Juglans regia Linn. by ultra performance liquid chromatography coupled with Orbitrap mass spectrometer. Food Chem. 2019; 286:669-677. DOI: 10.1016/j.foodchem.2019.02.054. View

Grace M, Warlick C, Neff S, Lila M . Efficient preparative isolation and identification of walnut bioactive components using high-speed counter-current chromatography and LC-ESI-IT-TOF-MS. Food Chem. 2014; 158:229-38. DOI: 10.1016/j.foodchem.2014.02.117. View

10.

Vallverdu-Queralt A, Boix N, Pique E, Gomez-Catalan J, Medina-Remon A, Sasot G . Identification of phenolic compounds in red wine extract samples and zebrafish embryos by HPLC-ESI-LTQ-Orbitrap-MS. Food Chem. 2015; 181:146-51. DOI: 10.1016/j.foodchem.2015.02.098. View

11.

Pandey K, Rizvi S . Plant polyphenols as dietary antioxidants in human health and disease. Oxid Med Cell Longev. 2010; 2(5):270-8. PMC: 2835915. DOI: 10.4161/oxim.2.5.9498. View

12.

Shimoda H, Tanaka J, Kikuchi M, Fukuda T, Ito H, Hatano T . Effect of polyphenol-rich extract from walnut on diet-induced hypertriglyceridemia in mice via enhancement of fatty acid oxidation in the liver. J Agric Food Chem. 2009; 57(5):1786-92. DOI: 10.1021/jf803441c. View

13.

Jahanban-Esfahlan A, Amarowicz R . Walnut ( L.) shell pyroligneous acid: chemical constituents and functional applications. RSC Adv. 2022; 8(40):22376-22391. PMC: 9081416. DOI: 10.1039/c8ra03684e. View

14.

Blum F . High performance liquid chromatography. Br J Hosp Med (Lond). 2014; 75(2):C18-21. DOI: 10.12968/hmed.2014.75.Sup2.C18. View

15.

Martinez M, Labuckas D, Lamarque A, Maestri D . Walnut (Juglans regia L.): genetic resources, chemistry, by-products. J Sci Food Agric. 2010; 90(12):1959-67. DOI: 10.1002/jsfa.4059. View

16.

Yamada H, Wakamori S, Hirokane T, Ikeuchi K, Matsumoto S . Structural Revisions in Natural Ellagitannins. Molecules. 2018; 23(8). PMC: 6222896. DOI: 10.3390/molecules23081901. View

17.

Saez V, Riquelme S, von Baer D, Vallverdu-Queralt A . Phenolic Profile of Grape Canes: Novel Compounds Identified by LC-ESI-LTQ-Orbitrap-MS. Molecules. 2019; 24(20). PMC: 6832258. DOI: 10.3390/molecules24203763. View

18.

Terra X, Pallares V, Ardevol A, Blade C, Fernandez-Larrea J, Pujadas G . Modulatory effect of grape-seed procyanidins on local and systemic inflammation in diet-induced obesity rats. J Nutr Biochem. 2010; 22(4):380-7. DOI: 10.1016/j.jnutbio.2010.03.006. View

19.

Leonetti D, Soleti R, Clere N, Vergori L, Jacques C, Duluc L . Extract Enriched in Flavan-3-ols and Mainly Procyanidin Dimers Improves Metabolic Alterations in a Mouse Model of Obesity-Related Disorders Partially via Estrogen Receptor Alpha. Front Pharmacol. 2018; 9:406. PMC: 5928481. DOI: 10.3389/fphar.2018.00406. View

20.

Hilary S, Tomas-Barberan F, Martinez-Blazquez J, Kizhakkayil J, Souka U, Al-Hammadi S . Polyphenol characterisation of Phoenix dactylifera L. (date) seeds using HPLC-mass spectrometry and its bioaccessibility using simulated in-vitro digestion/Caco-2 culture model. Food Chem. 2019; 311:125969. DOI: 10.1016/j.foodchem.2019.125969. View