Napthoquinones from Sp.-Antibiotic Activity Against , the Causative Agent of Bacterial Fruit Blotch in Watermelon and Melon

Overview

Journal J Fungi (Basel)

Date 2021 Jun 2

PMID 34066879

Citations 5

Authors

Anthikan Klomchit

Jorge Daniel Calderin

Wuttichai Jaidee

Kanchana Watla-Iad

Siraprapa Brooks

Affiliations

Soon will be listed here.

Abstract

Bacterial fruit blotch (BFB) is a bacterial disease that devastates crops worldwide, causing significant economic losses. Currently, there is no means to treat or control the disease. This study focused on exploring the antibacterial properties of endophytic fungi against (), the causative agent of BFB. Based on disc diffusion, time kill and MIC microdilution broth assays, four endophytes showed promise in controlling . Nonetheless, only one strain, sp. MFLUCC 17-0253, reduced the severity of disease on watermelon and melon seedlings up to 80%. Structure analysis revealed production of several compounds by the fungus. Three of these secondary metabolites, including mixture of 2-methoxy-6-methyl-7-acetonyl-8-hydroxy-1,4-maphthalenedione and 5,8-dihydroxy-7-acetonyl-1,4-naphthalenedione, anhydrojavanicin, and fusarnaphthoquinones B exhibited antagonistic activity against . The chemical profile data experiment analyzed by LC-Q/TOF-MS suggested successful colonization of endophytic fungi in their host plant and different metabolic profiles between treated and untreated seedling. Biofilm assay also demonstrated that secondary metabolites of sp. MFLUCC 17-0253 significantly inhibited biofilm development of . To the best of our knowledge, secondary metabolites that provide significant growth inhibition of are reported for the first time. Thus, sp. MFLUCC 17-0253 possesses high potential as a biocontrol agent for BFB disease.

Citing Articles

Soil fungal community structure and function response to rhizoma perennial peanut cultivars.

Daraz U, Erhunmwunse A, Dubeux Jr J, Mackowiak C, Liao H, Wang X BMC Plant Biol. 2024; 24(1):582.

PMID: 38898415 PMC: 11186081. DOI: 10.1186/s12870-024-05209-y.

, a stem rot disease in potato: Characterization, distribution and management.

Riaz M, Akhtar N, Msimbira L, Antar M, Ashraf S, Khan S Front Microbiol. 2022; 13:953097.

PMID: 36033873 PMC: 9403868. DOI: 10.3389/fmicb.2022.953097.

Synthesis of nano-sized lead sulfide thin films from Avocado (Glycosmis cochinchinensis) Leaf extracts to empower pollution remediation.

Saka A, Gudata L, Jule L, Seeivasan V, N N, Ramaswamy K Sci Rep. 2022; 12(1):11710.

PMID: 35810188 PMC: 9271032. DOI: 10.1038/s41598-022-15785-4.

Biological approach synthesis and characterization of iron sulfide (FeS) thin films from banana peel extract for contamination of environmental remediation.

Saka A, Jule L, Soressa S, Gudata L, Nagaprasad N, Seenivasan V Sci Rep. 2022; 12(1):10486.

PMID: 35729287 PMC: 9213450. DOI: 10.1038/s41598-022-14828-0.

Fungal Endophytes: A Potential Source of Antibacterial Compounds.

Deshmukh S, Dufosse L, Chhipa H, Saxena S, Mahajan G, Gupta M J Fungi (Basel). 2022; 8(2).

PMID: 35205918 PMC: 8877021. DOI: 10.3390/jof8020164.

References

Strobel G, Daisy B, Castillo U, Harper J . Natural products from endophytic microorganisms. J Nat Prod. 2004; 67(2):257-68. DOI: 10.1021/np030397v. View

Ueno Y, Sawano M, Ishii K . Production of trichothecene mycotoxins by Fusarium species in shake culture. Appl Microbiol. 1975; 30(1):4-9. PMC: 187104. DOI: 10.1128/am.30.1.4-9.1975. View

Burdman S, Walcott R . Acidovorax citrulli: generating basic and applied knowledge to tackle a global threat to the cucurbit industry. Mol Plant Pathol. 2012; 13(8):805-15. PMC: 6638624. DOI: 10.1111/j.1364-3703.2012.00810.x. View

Araujo W, Maccheroni Jr W, Barroso P, Saridakis H, Azevedo J . Variability and interactions between endophytic bacteria and fungi isolated from leaf tissues of citrus rootstocks. Can J Microbiol. 2001; 47(3):229-36. DOI: 10.1139/w00-146. View

Devi Bala B, Muthusaravanan S, Choon T, Ashraf Ali M, Perumal S . Sequential synthesis of amino-1,4-naphthoquinone-appended triazoles and triazole-chromene hybrids and their antimycobacterial evaluation. Eur J Med Chem. 2014; 85:737-46. DOI: 10.1016/j.ejmech.2014.08.009. View

Jiang C, Wu F, Yu Z, Xie P, Ke H, Li H . Study on screening and antagonistic mechanisms of Bacillus amyloliquefaciens 54 against bacterial fruit blotch (BFB) caused by Acidovorax avenae subsp. citrulli. Microbiol Res. 2014; 170:95-104. DOI: 10.1016/j.micres.2014.08.009. View

Dutta B, Avci U, Hahn M, Walcott R . Location of Acidovorax citrulli in infested watermelon seeds is influenced by the pathway of bacterial invasion. Phytopathology. 2012; 102(5):461-8. DOI: 10.1094/PHYTO-10-11-0286-R. View

Willems A, Goor M, Thielemans S, Gillis M, Kersters K, De Ley J . Transfer of several phytopathogenic Pseudomonas species to Acidovorax as Acidovorax avenae subsp. avenae subsp. nov., comb. nov., Acidovorax avenae subsp. citrulli, Acidovorax avenae subsp. cattleyae, and Acidovorax konjaci. Int J Syst Bacteriol. 1992; 42(1):107-19. DOI: 10.1099/00207713-42-1-107. View

Antonissen G, Martel A, Pasmans F, Ducatelle R, Verbrugghe E, Vandenbroucke V . The impact of Fusarium mycotoxins on human and animal host susceptibility to infectious diseases. Toxins (Basel). 2014; 6(2):430-52. PMC: 3942744. DOI: 10.3390/toxins6020430. View

10.

Johnson K, Minsavage G, Le T, Jones J, Walcott R . Efficacy of a Nonpathogenic Acidovorax citrulli Strain as a Biocontrol Seed Treatment for Bacterial Fruit Blotch of Cucurbits. Plant Dis. 2019; 95(6):697-704. DOI: 10.1094/PDIS-09-10-0660. View

11.

Khaw A, Sameni S, Venkatesan S, Kalthur G, Hande M . Plumbagin alters telomere dynamics, induces DNA damage and cell death in human brain tumour cells. Mutat Res Genet Toxicol Environ Mutagen. 2015; 793:86-95. DOI: 10.1016/j.mrgentox.2015.06.004. View

12.

Krishnamurthy Y, Naik S, Jayaram S . Fungal communities in herbaceous medicinal plants from the malnad region, southern India. Microbes Environ. 2011; 23(1):24-8. DOI: 10.1264/jsme2.23.24. View

13.

Ishii K, Sakai K, Ueno Y, Tsunoda H, Enomoto M . Solaniol, a toxic metabolite of Fusarium solani. Appl Microbiol. 1971; 22(4):718-20. PMC: 376392. DOI: 10.1128/am.22.4.718-720.1971. View

14.

Takemoto K, Kamisuki S, Chia P, Kuriyama I, Mizushina Y, Sugawara F . Bioactive dihydronaphthoquinone derivatives from Fusarium solani. J Nat Prod. 2014; 77(9):1992-6. DOI: 10.1021/np500175j. View

15.

Ribeiro L, Fumagalli F, Mello R, Froes T, da Silva M, Villamizar Gomez S . Structure-activity relationships and mechanism of action of tetragomycin derivatives as inhibitors of Staphylococcus aureus staphyloxanthin biosynthesis. Microb Pathog. 2020; 144:104127. DOI: 10.1016/j.micpath.2020.104127. View

16.

Werner R, APPEL K, Merk W . Gunacin, a new quinone antibiotic from Ustilago sp. J Antibiot (Tokyo). 1979; 32(11):1104-11. DOI: 10.7164/antibiotics.32.1104. View

17.

Imlay J . Pathways of oxidative damage. Annu Rev Microbiol. 2003; 57:395-418. DOI: 10.1146/annurev.micro.57.030502.090938. View

18.

Suzuki T, Kim Y, Yoshioka H, Iwahashi Y . Regulation of metabolic products and gene expression in Fusarium asiaticum by agmatine addition. Mycotoxin Res. 2013; 29(2):103-11. PMC: 3624000. DOI: 10.1007/s12550-013-0158-y. View

19.

BAUER A, KIRBY W, SHERRIS J, Turck M . Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966; 45(4):493-6. View

20.

Sarker S, Nahar L, Kumarasamy Y . Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods. 2007; 42(4):321-4. PMC: 1895922. DOI: 10.1016/j.ymeth.2007.01.006. View