CD73 Mesenchymal Stem Cells Ameliorate Myocardial Infarction by Promoting Angiogenesis

Overview

Journal Front Cell Dev Biol

Specialty Cell Biology

Date 2021 May 31

PMID 34055772

Citations 17

Authors

Qiong Li

Huifang Hou

Meng Li

Xia Yu

Hongbo Zuo

Jianhui Gao

Min Zhang

Zongjin Li

Zhikun Guo

Affiliations

Soon will be listed here.

Abstract

With multipotent differentiation potential and paracrine capacity, mesenchymal stem cells (MSCs) have been widely applied in clinical practice for the treatment of ischemic heart disease. MSCs are a heterogeneous population and the specific population of MSCs may exhibit a selective ability for tissue repair. The aim of our research was to adapt the CD73 subgroup of adipose derived MSCs (AD-MSCs) for the therapy of myocardial infarction (MI). In this research, AD-MSCs were isolated from adipose tissue surrounding the groin of mice and CD73 AD-MSCs were sorted using flow cytometry. To investigate the therapeutic effects of CD73 AD-MSCs, 1.2 × 10 CD73 AD-MSCs were transplanted into rat model of MI, and CD73 AD-MSCs, normal AD-MSCs transplantation served as control. Our results revealed that CD73 AD-MSCs played a more effective role in the acceleration function of cardiac recovery by promoting angiogenesis in a rat model of MI compared with mixed AD-MSCs and CD73 AD-MSCs. Moreover, with the expression of CD73 in AD-MSCs, the secretion of VEGF, SDF-1α, and HGF factors could be promoted. It also shows differences between CD73 and CD73 AD-MSCs when the transcription profiles of these two subgroups were compared, especially in VEGF pathway. These findings raise an attractive outlook on CD73 AD-MSCs as a dominant subgroup for treating MI-induced myocardial injury. CD73, a surface marker, can be used as a MSCs cell quality control for the recovery of MI by accelerating angiogenesis.

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