16S RDNA Full-Length Assembly Sequencing Technology Analysis of Intestinal Microbiome in Polycystic Ovary Syndrome

Overview

Journal Front Cell Infect Microbiol

Specialties Cell Biology
Infectious Diseases
Microbiology

Date 2021 May 27

PMID 34041041

Citations 25

Authors

Sitong Dong

Jiao Jiao

Shuangshuo Jia

Gaoyu Li

Wei Zhang

Kai Yang

Zhen Wang

Chao Liu

Da Li

Xiuxia Wang

Affiliations

Soon will be listed here.

Abstract

Objective: To study the characteristics and relationship of the gut microbiota in patients with polycystic ovary syndrome (PCOS).

Method: We recruited 45 patients with PCOS and 37 healthy women from the Reproductive Department of Shengjing Hospital. We recorded their clinical indexes, and sequenced their fecal samples by 16S rDNA full-length assembly sequencing technology (16S-FAST).

Result: We found decreased α diversity and different abundances of a series of microbial species in patients with PCOS compared to healthy controls. We found LH and AMH were significantly increased in PCOS with enterotype when compared to control women with enterotype, while glucose and lipid metabolism level remained no significant difference, and situations were opposite in PCOS and control women with enterotype. and were more abundant while spp. were less abundant in the PCOS group. was significantly more prevalent in PCOS-not insulin resistance (NIR) compared to control-NIR and PCOS-not overweight (NOW) patient groups compared to control-NOW groups. Kyoto Encyclopedia Genes and Genomes reflecting pathways related to lipopolysaccharide biosynthesis were more abundant in the PCOS group.

Conclusion: Our study found gut microbiota that had different abundance in patients with PCOS compared to healthy controls. An intimate relationship was shown between the gut microbiota and pathological changes in PCOS. We suggest the gut microbiota should be taken into consideration in the treatment of symptoms of PCOS drugs and diet.

Citing Articles

Fecal propionate is a signature of insulin resistance in polycystic ovary syndrome.

Dong S, Yao X, Jiao J, Lin B, Yan F, Wang X Front Cell Infect Microbiol. 2025; 14:1394873.

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Signature of pre-pregnancy microbiome in infertile women undergoing frozen embryo transfer with gestational diabetes mellitus.

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Effectiveness of Probiotics, Prebiotics, and Synbiotics in Managing Insulin Resistance and Hormonal Imbalance in Women with Polycystic Ovary Syndrome (PCOS): A Systematic Review of Randomized Clinical Trials.

Martinez Guevara D, Vidal Canas S, Palacios I, Gomez A, Estrada M, Gallego J Nutrients. 2024; 16(22).

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Gut microbiota in women with polycystic ovary syndrome: an individual based analysis of publicly available data.

Yang Y, Cheng J, Liu C, Zhang X, Ma N, Zhou Z EClinicalMedicine. 2024; 77:102884.

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Gut Microbiota and Oral Contraceptive Use in Women with Polycystic Ovary Syndrome: A Systematic Review.

Wydra J, Szlendak-Sauer K, Zgliczynska M, Zeber-Lubecka N, Ciebiera M Nutrients. 2024; 16(19).

PMID: 39408349 PMC: 11478613. DOI: 10.3390/nu16193382.

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