Co-culture of Human Intestinal Organoids and Lamina Propria-derived CD4 T cells

Overview

Journal STAR Protoc

Publisher Cell Press

Specialties Biology
Biomedical Engineering
Science

Date 2021 May 26

PMID 34036282

Citations 13

Authors

Renee R C E Schreurs

Martin E Baumdick

Agata Drewniak

Madeleine J Bunders

Affiliations

Soon will be listed here.

Abstract

Crosstalk between immune cells and intestinal stem cells (ISCs) plays a critical role in tissue homeostasis and inflammation; however, models based on primary cells recapitulating this interaction were lacking. Here, we provide a detailed protocol for an autologous long-term 3D co-culture system of human intestinal CD4 T cells and ISCs to study T cell-intestinal epithelial cell interactions during tissue development and inflammation. For complete details on the use and execution of this protocol, please refer to Schreurs et al. (2019).

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