Recent Advances in Cell-Based In Vitro Models to Recreate Human Intestinal Inflammation

Overview

Journal Adv Sci (Weinh)

Specialty Biomedical Engineering

Date 2023 Sep 22

PMID 37736674

Authors

Maria Helena Macedo

Mafalda Dias Neto

Lorenzo Pastrana

Catarina Goncalves

Miguel Xavier

Affiliations

Soon will be listed here.

Abstract

Inflammatory bowel disease causes a major burden to patients and healthcare systems, raising the need to develop effective therapies. Technological advances in cell culture, allied with ethical issues, have propelled in vitro models as essential tools to study disease aetiology, its progression, and possible therapies. Several cell-based in vitro models of intestinal inflammation have been used, varying in their complexity and methodology to induce inflammation. Immortalized cell lines are extensively used due to their long-term survival, in contrast to primary cultures that are short-lived but patient-specific. Recently, organoids and organ-chips have demonstrated great potential by being physiologically more relevant. This review aims to shed light on the intricate nature of intestinal inflammation and cover recent works that report cell-based in vitro models of human intestinal inflammation, encompassing diverse approaches and outcomes.

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