Increased AOC1 Expression Promotes Cancer Progression in Colorectal Cancer

Background: Amine oxidase copper containing 1 () is a gene whose biological function in colorectal cancer (CRC) has not been elucidated. Therefore, the purpose of this study was to investigate the clinical significance of expression in CRC and its biological function in CRC cell lines.

Materials And Methods: expression levels were examined in paired CRC and peritumoral tissues, and distant liver metastatic tissues were examined using quantitative real-time PCR, western blotting, and immunohistochemistry staining. The log-rank test and Cox regression model were used to analyze the relationship between expression and prognosis. Proliferation assays (Cell Counting Kit-8 and colony formation assays), migration assays (Transwell and wound healing assays) and xenograft tumor formation in nude mice were performed to assess the biological role of in CRC cells.

Results: expression significantly increased in human CRC tissues, especially in liver metastases, and was associated with a worse prognosis. In addition, had higher expression in tumor organoids than in normal organoids, suggesting that it was highly expressed in the tumor epithelium. Functional analysis demonstrated that knockdown inhibited the proliferation and migration of CRC cells by inducing EMT . Xenograft tumor formation in nude mice showed that knockdown of inhibited the tumor xenografts growth .

Conclusion: High expression of was significantly associated with worse clinical outcomes, was an independent risk factor for poor prognosis, and promoted aggressive CRC cell phenotypes. is expected to become a novel biomarker for predicting the prognosis of patients with CRC and an effective therapeutic target in clinical practice.