MiR-720 is a Key Regulator of Glioma Migration and Invasion by Controlling TARSL2 Expression

Overview

Journal Hum Cell

Publisher Springer

Specialty Cell Biology

Date 2021 May 23

PMID 34024034

Citations 6

Authors

Yinlong Liu

Kuan Jiang

Tongle Zhi

Xiupeng Xu

Affiliations

Soon will be listed here.

Abstract

Glioblastoma (GBM) is the most lethal type of primary brain tumor and is characterized by diffuse infiltrative growth. However, the mechanisms that control this phenotype remain largely unknown. Emerging evidence has demonstrated that the abnormal expression of microRNAs and their target genes are involved in the migration and invasion of glioma cells. In this study, we demonstrated that microRNA-720 (miR-720) was significantly upregulated in glioma tissues and cells. Functional experiments showed that overexpression of miR-720 promotes glioma migration and invasion, while downregulation of miR-720 inhibits glioma migration and invasion. Meanwhile, we found that threonyl-tRNA synthetase like-2 (TARSL2) was a direct and functional target of miR-720 in glioma. Reintroduction of TARSL2 into glioma cells repressed the invasion promoting function of miR-720, whereas downregulation of TARSL2 reversed the anti-invasion function of anti-miR-720. Furthermore, quantitative real-time polymerase chain reaction results showed that miR-720 was inversely correlated with TARSL2 expression in 40 GBM tissues. Finally, in vivo experiments showed that miR-720 promotes glioma growth and upregulates invasion-related genes in nude mice. Overall, our findings suggest increasing miR-720 enhances glioma migration and invasion through downregulation of TARSL2, which may provide novel insight into the treatment of glioma.

Citing Articles

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References

Wen P, Kesari S . Malignant gliomas in adults. N Engl J Med. 2008; 359(5):492-507. DOI: 10.1056/NEJMra0708126. View

Onishi M, Ichikawa T, Kurozumi K, Date I . Angiogenesis and invasion in glioma. Brain Tumor Pathol. 2011; 28(1):13-24. DOI: 10.1007/s10014-010-0007-z. View

Rupaimoole R, Slack F . MicroRNA therapeutics: towards a new era for the management of cancer and other diseases. Nat Rev Drug Discov. 2017; 16(3):203-222. DOI: 10.1038/nrd.2016.246. View

Li X, Kong S, Cao Y . miR-1254 inhibits progression of glioma in vivo and in vitro by targeting CSF-1. J Cell Mol Med. 2020; 24(5):3128-3138. PMC: 7077535. DOI: 10.1111/jcmm.14981. View

Zeng A, Yin J, Wang Z, Zhang C, Li R, Zhang Z . miR-17-5p-CXCL14 axis related transcriptome profile and clinical outcome in diffuse gliomas. Oncoimmunology. 2018; 7(12):e1510277. PMC: 6279316. DOI: 10.1080/2162402X.2018.1510277. View

Zhang R, Luo H, Wang S, Chen W, Chen Z, Wang H . MicroRNA-377 inhibited proliferation and invasion of human glioblastoma cells by directly targeting specificity protein 1. Neuro Oncol. 2014; 16(11):1510-22. PMC: 4201073. DOI: 10.1093/neuonc/nou111. View

Kawachi H, Sugahara K, Nakamura Y, Katakura A, Minaguchi K, Shibahara T . Deletion polymorphism at chromosome 3q26.1 and oral squamous cell carcinoma. Int J Oncol. 2012; 42(2):384-90. PMC: 3583626. DOI: 10.3892/ijo.2012.1749. View

Bhat N, Colden M, Dar A, Saini S, Arora P, Shahryari V . MicroRNA-720 Regulates E-cadherin-αE-catenin Complex and Promotes Renal Cell Carcinoma. Mol Cancer Ther. 2017; 16(12):2840-2848. PMC: 5893503. DOI: 10.1158/1535-7163.MCT-17-0400. View

10.

Tang Y, Lin Y, Li C, Hu X, Liu Y, He M . MicroRNA-720 promotes in vitro cell migration by targeting Rab35 expression in cervical cancer cells. Cell Biosci. 2015; 5:56. PMC: 4583841. DOI: 10.1186/s13578-015-0047-5. View

11.

Wang X, Kuang Y, Shen X, Zhou H, Chen Y, Han Y . Evaluation of miR-720 prognostic significance in patients with colorectal cancer. Tumour Biol. 2014; 36(2):719-27. DOI: 10.1007/s13277-014-2697-z. View

12.

Shinozuka E, Miyashita M, Mizuguchi Y, Akagi I, Kikuchi K, Makino H . SnoN/SKIL modulates proliferation through control of hsa-miR-720 transcription in esophageal cancer cells. Biochem Biophys Res Commun. 2012; 430(1):101-6. DOI: 10.1016/j.bbrc.2012.11.005. View

13.

Li L, Zhang C, Liu L, Yi C, Lu S, Zhou X . miR-720 inhibits tumor invasion and migration in breast cancer by targeting TWIST1. Carcinogenesis. 2013; 35(2):469-78. DOI: 10.1093/carcin/bgt330. View

14.

Nie A, Sun B, Fu Z, Yu D . Roles of aminoacyl-tRNA synthetases in immune regulation and immune diseases. Cell Death Dis. 2019; 10(12):901. PMC: 6883034. DOI: 10.1038/s41419-019-2145-5. View

15.

Kwon N, Fox P, Kim S . Aminoacyl-tRNA synthetases as therapeutic targets. Nat Rev Drug Discov. 2019; 18(8):629-650. DOI: 10.1038/s41573-019-0026-3. View

16.

Hyeon D, Kim J, Ahn T, Cho Y, Hwang D, Kim S . Evolution of the multi-tRNA synthetase complex and its role in cancer. J Biol Chem. 2019; 294(14):5340-5351. PMC: 6462501. DOI: 10.1074/jbc.REV118.002958. View

17.

Kim K, Park S, Na S, Kim J, Choi H, Kim Y . Reinvestigation of aminoacyl-tRNA synthetase core complex by affinity purification-mass spectrometry reveals TARSL2 as a potential member of the complex. PLoS One. 2013; 8(12):e81734. PMC: 3846882. DOI: 10.1371/journal.pone.0081734. View

18.

Kim S, Kim H, Park M, Cho S, Goughnour P, Han D . Caspase-8 controls the secretion of inflammatory lysyl-tRNA synthetase in exosomes from cancer cells. J Cell Biol. 2017; 216(7):2201-2216. PMC: 5496609. DOI: 10.1083/jcb.201605118. View

19.

Nam S, Kang M, Ryu J, Kim H, Kim D, Kim D . Suppression of lysyl-tRNA synthetase, KRS, causes incomplete epithelial-mesenchymal transition and ineffective cell‑extracellular matrix adhesion for migration. Int J Oncol. 2016; 48(4):1553-60. DOI: 10.3892/ijo.2016.3381. View

20.

Nam S, Kim D, Lee D, Lee H, Song D, Jung J . Lysyl-tRNA synthetase-expressing colon spheroids induce M2 macrophage polarization to promote metastasis. J Clin Invest. 2018; 128(11):5034-5055. PMC: 6205414. DOI: 10.1172/JCI99806. View

21.

Li C, Zheng H, Hou W, Bao H, Xiong J, Che W . Long non-coding RNA linc00645 promotes TGF-β-induced epithelial-mesenchymal transition by regulating miR-205-3p-ZEB1 axis in glioma. Cell Death Dis. 2019; 10(10):717. PMC: 6763487. DOI: 10.1038/s41419-019-1948-8. View