Naringin-inlaid Silk Fibroin/hydroxyapatite Scaffold Enhances Human Umbilical Cord-derived Mesenchymal Stem Cell-based Bone Regeneration

Overview

Journal Cell Prolif

Date 2021 May 19

PMID 34008897

Citations 21

Authors

Zhi-Hu Zhao

Xin-Long Ma

Bin Zhao

Peng Tian

Jian-Xiong Ma

Jia-Yu Kang

Yang Zhang

Yue Guo

Lei Sun

Affiliations

Soon will be listed here.

Abstract

Objectives: Large bone defects are a common, debilitating clinical condition that have substantial global health and economic burden. Bone tissue engineering technology has become one of the most promising approaches for regenerating defective bones. In this study, we fabricated a naringin-inlaid composite silk fibroin/hydroxyapatite (NG/SF/HAp) scaffold to repair bone defects.

Materials And Methods: The salt-leaching technology was used to fabricate the NG/SF/HAp scaffold. The cytocompatibility of the NG/SF/HAp scaffold was assessed using scanning electron microscopy, live/dead cell staining and phalloidin staining. The osteogenic and angiogenic properties were assessed in vitro and in vivo.

Results: The porous NG/SF/HAp scaffold had a well-designed biomimetic porous structure with osteoinductive and angiogenic activities. A gene microarray identified 854 differentially expressed genes between human umbilical cord-derived mesenchymal stem cells (hUCMSCs) cultured on SF-nHAp scaffolds and cells cultured on NG/SF/HAp scaffolds. The underlying osteoblastic mechanism was investigated using hUCMSCs in vitro. Naringin facilitated hUCMSC ingrowth into the SF/HAp scaffold and promoted osteogenic differentiation. The osteogenic and angiogenic capabilities of cells cultured in the NG/SF/HAp scaffold were superior to those of cells cultured in the SF/HAp scaffold.

Conclusions: The data indicate the potential of the SF/HAp composite scaffold incorporating naringin for bone regeneration.

Citing Articles

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Resorbable Membranes for Guided Bone Regeneration: Critical Features, Potentials, and Limitations.

Abtahi S, Chen X, Shahabi S, Nasiri N ACS Mater Au. 2023; 3(5):394-417.

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Flavonoid-Loaded Biomaterials in Bone Defect Repair.

Yang J, Zhang L, Ding Q, Zhang S, Sun S, Liu W Molecules. 2023; 28(19).

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