Platelet Number and Function in Response to a Single Intravenous Dose of Vincristine

Overview

Journal J Vet Intern Med

Specialties General Medicine
Veterinary Medicine

Date 2021 May 16

PMID 33993531

Citations 4

Authors

Erin C Allen

Jaime L Tarigo

Dana N LeVine

Jamie P Barber

Benjamin M Brainard

Affiliations

Soon will be listed here.

Abstract

Background: Vincristine might increase circulating platelet numbers but the functional capacity of these newly released platelets is unknown.

Objective: To evaluate and compare the functionality of mature and immature (reticulated) platelets after a single intravenous dose of vincristine in dogs.

Animals: Ten healthy purpose-bred dogs.

Methods: Dogs prospectively received a single IV injection of 0.02 mg/kg vincristine or 0.9% saline. Before and after treatment on days 3, 5, and 7, platelets (resting and after thrombin stimulation) were assessed by flow cytometric determination of P-selectin (CD62P) expression. Reticulated platelets were distinguished using thiazole orange (TO) staining.

Results: Relative to saline, vincristine administration increased platelet count from day 0 to day 7 (225 ± 58 to 273 ± 65 × 10 /μL, vs 299 ± 76.4 to 214 ± 20 × 10 /μL, P = .01) and increased percentage of reticulated platelets from day 0 to day 5 (3.9 ± 1.5% to 6.1 ± 1.6%, P = .02). On all days, reticulated platelets had greater resting expression of CD62P than did mature platelets (49.6 ± 4% vs 10.2 ± 1%, P ≤ .001). Across all days, CD62P expression by reticulated platelets in the vincristine and saline-treated groups was not different when unstimulated (P = .7) or after thrombin stimulation (P = .33).

Conclusions And Clinical Importance: Reticulated platelets released in response to vincristine administration function similarly to mature platelets.

Citing Articles

Evaluation of activation characteristics of a canine platelet concentrate produced by a commercial double centrifugation system.

Tey N, Koenig A, Hodges K, Brainard B Front Vet Sci. 2024; 11:1384938.

PMID: 38855414 PMC: 11157093. DOI: 10.3389/fvets.2024.1384938.

ACVIM consensus statement on the treatment of immune thrombocytopenia in dogs and cats.

LeVine D, Goggs R, Kohn B, Mackin A, Kidd L, Garden O J Vet Intern Med. 2024; 38(4):1982-2007.

PMID: 38779941 PMC: 11256181. DOI: 10.1111/jvim.17079.

Influence of Vincristine, Clinically Used in Cancer Therapy and Immune Thrombocytopenia, on the Function of Human Platelets.

Lien L, Lu W, Lin K, Kang L, Chen T, Lin B Molecules. 2021; 26(17).

PMID: 34500771 PMC: 8434001. DOI: 10.3390/molecules26175340.

Platelet number and function in response to a single intravenous dose of vincristine.

Allen E, Tarigo J, LeVine D, Barber J, Brainard B J Vet Intern Med. 2021; 35(4):1754-1762.

PMID: 33993531 PMC: 8295686. DOI: 10.1111/jvim.16169.

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