Circular RNA CircFADS2 is Overexpressed in Sepsis and Suppresses LPS-induced Lung Cell Apoptosis by Inhibiting the Maturation of MiR-15a-5p

Overview

Journal BMC Immunol

Publisher Biomed Central

Specialty Allergy & Immunology

Date 2021 May 13

PMID 33980140

Citations 10

Authors

Xiaoyang Hong

Shuanglei Li

Jie Wang

Zhe Zhao

Zhichun Feng

Affiliations

Soon will be listed here.

Abstract

Background: Circular RNA circFADS2 plays protective roles in LPS-induced inflammation, which promotes sepsis, suggesting its involvement in sepsis.

Methods: Expression of circFADS2, mature miR-15a-5p, and miR-15a-5p precursor in plasma samples from sepsis patients and healthy controls was determined by RT-qPCR. The circFADS2 expression vector was transfected in lung cells, followed by the measurement of the expression levels of mature miR-15a-5p and miR-15a-5p precursor to study the role of circFADS2 in miR-15a-5p maturation. Cell apoptosis was analyzed by cell apoptosis assay.

Results: CircFADS2 was upregulated in sepsis and inversely correlated with mature miR-15a-5p, but not miR-15a-5p precursor. In lung cells, circFADS2 overexpression decreased the level of mature miR-15a-5p, but not miR-15a-5p precursor. LPS treatment decreased miR-15a-5p expression and increased circFADS2 level. Cell apoptosis analysis showed that circFADS2 overexpression reduced miR-15a-5p overexpression-induced apoptosis of LPS-treated lung cells.

Conclusions: CircFADS2 is upregulated in sepsis to suppress LPS-induced lung cell apoptosis by inhibiting miR-15a-5p maturation.

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References

Hotchkiss R, Moldawer L, Opal S, Reinhart K, Turnbull I, Vincent J . Sepsis and septic shock. Nat Rev Dis Primers. 2017; 2:16045. PMC: 5538252. DOI: 10.1038/nrdp.2016.45. View

Haque S, Harries L . Circular RNAs (circRNAs) in Health and Disease. Genes (Basel). 2017; 8(12). PMC: 5748671. DOI: 10.3390/genes8120353. View

Ames S, Davis B, Angus D, Carcillo J, Kahn J . Hospital Variation in Risk-Adjusted Pediatric Sepsis Mortality. Pediatr Crit Care Med. 2018; 19(5):390-396. PMC: 5935525. DOI: 10.1097/PCC.0000000000001502. View

Prescott H, Angus D . Enhancing Recovery From Sepsis: A Review. JAMA. 2018; 319(1):62-75. PMC: 5839473. DOI: 10.1001/jama.2017.17687. View

Seymour C, Gesten F, Prescott H, Friedrich M, Iwashyna T, Phillips G . Time to Treatment and Mortality during Mandated Emergency Care for Sepsis. N Engl J Med. 2017; 376(23):2235-2244. PMC: 5538258. DOI: 10.1056/NEJMoa1703058. View

Xiao Y, Tong H, Yuan X, Xiong C, Xu X, Zeng Y . : A potential prognostic biomarker of colorectal cancer. Exp Biol Med (Maywood). 2020; 245(14):1233-1241. PMC: 7437371. DOI: 10.1177/1535370220929965. View

Beltran-Garcia J, Osca-Verdegal R, Nacher-Sendra E, Pallardo F, Garcia-Gimenez J . Circular RNAs in Sepsis: Biogenesis, Function, and Clinical Significance. Cells. 2020; 9(6). PMC: 7349763. DOI: 10.3390/cells9061544. View

Bosmann M, Ward P . The inflammatory response in sepsis. Trends Immunol. 2012; 34(3):129-36. PMC: 3543471. DOI: 10.1016/j.it.2012.09.004. View

Kaukonen K, Bailey M, Pilcher D, Cooper D, Bellomo R . Systemic inflammatory response syndrome criteria in defining severe sepsis. N Engl J Med. 2015; 372(17):1629-38. DOI: 10.1056/NEJMoa1415236. View

10.

Zhao F, Han Y, Liu Z, Zhao Z, Li Z, Jia K . circFADS2 regulates lung cancer cells proliferation and invasion via acting as a sponge of miR-498. Biosci Rep. 2018; 38(4). PMC: 6117614. DOI: 10.1042/BSR20180570. View

11.

Zeng L, Kang R, Zhu S, Wang X, Cao L, Wang H . ALK is a therapeutic target for lethal sepsis. Sci Transl Med. 2017; 9(412). PMC: 5737927. DOI: 10.1126/scitranslmed.aan5689. View

12.

Li R, Guo C, Li Y, Qin Z, Huang W . Therapeutic targets and signaling mechanisms of vitamin C activity against sepsis: a bioinformatics study. Brief Bioinform. 2020; 22(3). PMC: 7454291. DOI: 10.1093/bib/bbaa079. View

13.

Hung Y, Fang S, Wang S, Cheng W, Liu P, Su C . Corylin protects LPS-induced sepsis and attenuates LPS-induced inflammatory response. Sci Rep. 2017; 7:46299. PMC: 5387730. DOI: 10.1038/srep46299. View

14.

Li G, Tan W, Fang Y, Wu X, Zhou W, Zhang C . circFADS2 protects LPS-treated chondrocytes from apoptosis acting as an interceptor of miR-498/mTOR cross-talking. Aging (Albany NY). 2019; 11(10):3348-3361. PMC: 6555446. DOI: 10.18632/aging.101986. View

15.

Petronilho F, Florentino D, Danielski L, Vieira L, Martins M, Vieira A . Alpha-Lipoic Acid Attenuates Oxidative Damage in Organs After Sepsis. Inflammation. 2015; 39(1):357-365. DOI: 10.1007/s10753-015-0256-4. View

16.

Zhang W, Li Y, Xi X, Zhu G, Wang S, Liu Y . MicroRNA‑15a‑5p induces pulmonary artery smooth muscle cell apoptosis in a pulmonary arterial hypertension model via the VEGF/p38/MMP‑2 signaling pathway. Int J Mol Med. 2020; 45(2):461-474. PMC: 6984778. DOI: 10.3892/ijmm.2019.4434. View

17.

Bauer M, Coldewey S, Leitner M, Loffler B, Weis S, Wetzker R . Deterioration of Organ Function As a Hallmark in Sepsis: The Cellular Perspective. Front Immunol. 2018; 9:1460. PMC: 6028602. DOI: 10.3389/fimmu.2018.01460. View