Serum Levels of Soluble Fas and Fas Ligand in Iranian Women with Pre-eclampsia

Overview

Journal Rep Biochem Mol Biol

Specialty Biochemistry

Date 2021 May 10

PMID 33969132

Citations 1

Authors

Elham Masoumi

Asad Mirzaei

Haniyeh Ghaffari-Nazari

Sahar Tahaghoghi-Hajghorbani

Seyed Amir Jalali

Jalil Tavakkol-Afshari

Affiliations

Soon will be listed here.

Abstract

Background: The precise responsible mechanism of pre-eclampsia remains controversial however, recent data suggest a main role of the abnormal activation of the adaptive immune system and Apoptosis. In this study, we have measured serum levels of Fas/Fasl as two important members of extrinsic apoptotic pathway in patient with pre-eclampsia.

Methods: 207 participants including 99 pre-eclampsia patients and 108 age and sex-matched normal pregnant women were involved in the case-control study. Plasma sample from each participant was collected and stored at -20 °C until batch processing.Serum levels of Fas and Fas ligand were measured by ELISA for each participant including 99 pre-eclampsia patients and 108 normal pregnant women. Following a test of statistical normality, nonparametric data were analyzed by Mann-Whitney.

Results: sFas levels in case group was significantly higher than controls; 584 (397-892) pg/ml in cases opposed to 341 (213-602) pg/ml in controls (p value< 0.01). sFasL in pre-eclampsia women was a little lower than controls; 255 (173-318) pg/ml and in case group compared to 265.5 (184-381.5) pg/ml in controls.

Conclusion: We have found the increased levels of sFas in patients with pre-eclampsia in compare with the healthy pregnant women. It seems that abnormality in sFAS is related with pre-eclampsia.

Citing Articles

A New Method to Estimate Inhibition Percentage of Endogenous Digitalis in Patients with Pre-eclampsia.

Saad Al-Kawaz H, Yasser O, Mousa M Rep Biochem Mol Biol. 2022; 11(1):138-145.

PMID: 35765519 PMC: 9208553. DOI: 10.52547/rbmb.11.1.138.

References

Moffett A, Hiby S . How Does the maternal immune system contribute to the development of pre-eclampsia?. Placenta. 2007; 28 Suppl A:S51-6. DOI: 10.1016/j.placenta.2006.11.008. View

Hsu C, Harirah H, Basherra H, Mor G . Serum soluble Fas levels in preeclampsia. Obstet Gynecol. 2001; 97(4):530-2. DOI: 10.1016/s0029-7844(00)01227-8. View

Mellor A, Munn D . Immunology at the maternal-fetal interface: lessons for T cell tolerance and suppression. Annu Rev Immunol. 2000; 18:367-91. DOI: 10.1146/annurev.immunol.18.1.367. View

Ramsdell F, Seaman M, Miller R, Picha K, Kennedy M, Lynch D . Differential ability of Th1 and Th2 T cells to express Fas ligand and to undergo activation-induced cell death. Int Immunol. 1994; 6(10):1545-53. DOI: 10.1093/intimm/6.10.1545. View

Jalali S, Homaei Shandiz F, Tavakol Afshari J, Davarpanah Tanha Ghochan M, Nikpoor A, Mohammadi M . Status of FAS and FAS Ligand Gene Polymorphisms in Patients with Breast Cancer in Northeastern IRAN. Rep Biochem Mol Biol. 2018; 7(1):23-29. PMC: 6175584. View

Uckan D, Steele A, Cherry , Wang B, Chamizo W, Koutsonikolis A . Trophoblasts express Fas ligand: a proposed mechanism for immune privilege in placenta and maternal invasion. Mol Hum Reprod. 1997; 3(8):655-62. DOI: 10.1093/molehr/3.8.655. View

Darmochwal-Kolarz D, Leszczynska-Gorzelak B, Rolinski J, Oleszczuk J . T helper 1- and T helper 2-type cytokine imbalance in pregnant women with pre-eclampsia. Eur J Obstet Gynecol Reprod Biol. 1999; 86(2):165-70. DOI: 10.1016/s0301-2115(99)00065-2. View

Abrahams V, Straszewski-Chavez S, Guller S, Mor G . First trimester trophoblast cells secrete Fas ligand which induces immune cell apoptosis. Mol Hum Reprod. 2003; 10(1):55-63. DOI: 10.1093/molehr/gah006. View

Musial K, Zwolinska D . Matrix metalloproteinases and soluble Fas/FasL system as novel regulators of apoptosis in children and young adults on chronic dialysis. Apoptosis. 2011; 16(7):653-9. PMC: 3098372. DOI: 10.1007/s10495-011-0604-2. View

10.

Li N, Nie H, Yu Q, Zhang J, Ma A, Shen B . Role of soluble Fas ligand in autoimmune diseases. World J Gastroenterol. 2004; 10(21):3151-6. PMC: 4611260. DOI: 10.3748/wjg.v10.i21.3151. View

11.

Laskowska M, Laskowska K, Leszczynska-Gorzelak B, Oleszczuk J . Evaluation of the maternal and umbilical vein serum sFas/sFasL system in pregnancies complicated by preeclampsia with intrauterine growth retardation. Eur J Obstet Gynecol Reprod Biol. 2005; 126(2):155-9. DOI: 10.1016/j.ejogrb.2005.08.015. View

12.

Nagata S . Fas and Fas ligand: a death factor and its receptor. Adv Immunol. 1994; 57:129-44. DOI: 10.1016/s0065-2776(08)60672-0. View

13.

Roh C, Lee J, Kang B, Yang S, Kim B, Bae D . Differential expressions of Fas and Fas ligand in human placenta. J Korean Med Sci. 2002; 17(2):213-6. PMC: 3054862. DOI: 10.3346/jkms.2002.17.2.213. View

14.

Zhang X, Brunner T, Carter L, Dutton R, Rogers P, Bradley L . Unequal death in T helper cell (Th)1 and Th2 effectors: Th1, but not Th2, effectors undergo rapid Fas/FasL-mediated apoptosis. J Exp Med. 1997; 185(10):1837-49. PMC: 2196321. DOI: 10.1084/jem.185.10.1837. View

15.

Ciarmela P, Boschi S, Bloise E, Marozio L, Benedetto C, Castellucci M . Polymorphisms of FAS and FAS ligand genes in preeclamptic women. Eur J Obstet Gynecol Reprod Biol. 2009; 148(2):144-6. DOI: 10.1016/j.ejogrb.2009.10.026. View

16.

Enjoji M, Yamaguchi K, Nakashima M, Ohta S, Kotoh K, Fukushima M . Serum levels of soluble molecules associated with evasion of immune surveillance: a study in biliary disease. Liver Int. 2004; 24(4):330-4. DOI: 10.1111/j.1478-3231.2004.0931.x. View

17.

Saito S, Sakai M . Th1/Th2 balance in preeclampsia. J Reprod Immunol. 2003; 59(2):161-73. DOI: 10.1016/s0165-0378(03)00045-7. View

18.

Darmochwal-Kolarz D, Leszczynska-Gorzelak B, Rolinski J, Oleszczuk J . The expression and concentrations of Fas/APO-1 (CD95) antigen in patients with severe pre-eclampsia. J Reprod Immunol. 2001; 49(2):153-64. DOI: 10.1016/s0165-0378(00)00086-3. View

19.

Hoshimoto K, Hayashi M, Ohkura T . Plasma levels of soluble Fas during normal pregnancy. Gynecol Obstet Invest. 2001; 51(2):96-8. DOI: 10.1159/000052901. View

20.

Masoumi E, Tavakkol-Afshari J, Nikpoor A, Ghaffari-Nazari H, Tahaghoghi-Hajghorbani S, Jalali S . Relationship between Fas and Fas Ligand gene polymorphisms and pre-eclampsia. J Obstet Gynaecol Res. 2016; 42(10):1272-1278. DOI: 10.1111/jog.13062. View