Imaging Developmental Cell Cycles

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 2021 May 8

PMID 33964274

Citations 4

Authors

Abraham Q Kohrman

Rebecca P Kim-Yip

Eszter Posfai

Affiliations

Soon will be listed here.

Abstract

The last decade has seen a major expansion in development of live biosensors, the tools needed to genetically encode them into model organisms, and the microscopic techniques used to visualize them. When combined, these offer us powerful tools with which to make fundamental discoveries about complex biological processes. In this review, we summarize the availability of biosensors to visualize an essential cellular process, the cell cycle, and the techniques for single-cell tracking and quantification of these reporters. We also highlight studies investigating the connection of cellular behavior to the cell cycle, particularly through live imaging, and anticipate exciting discoveries with the combination of these technologies in developmental contexts.

Citing Articles

Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals.

Gest A, Sahan A, Zhong Y, Lin W, Mehta S, Zhang J Chem Rev. 2024; 124(22):12573-12660.

PMID: 39535501 PMC: 11613326. DOI: 10.1021/acs.chemrev.4c00293.

Two coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression.

Ando R, Sakaue-Sawano A, Shoda K, Miyawaki A Cell Struct Funct. 2023; 48(2):135-144.

PMID: 37394513 PMC: 10958192. DOI: 10.1247/csf.23028.

CDK activity sensors: genetically encoded ratiometric biosensors for live analysis of the cell cycle.

Martinez M, Matus D Biochem Soc Trans. 2022; 50(3):1081-1090.

PMID: 35674434 PMC: 9661961. DOI: 10.1042/BST20211131.

Cell cycle control during early embryogenesis.

Brantley S, Di Talia S Development. 2021; 148(13).

PMID: 34164654 PMC: 8255047. DOI: 10.1242/dev.193128.

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