Circular RNA CiRS-7 Affects the Propagation of Cryptosporidium Parvum in HCT-8 Cells by Sponging MiR-1270 to Activate the NF-κB Signaling Pathway

Overview

Journal Parasit Vectors

Publisher Biomed Central

Specialties Parasitology
Tropical Medicine

Date 2021 May 7

PMID 33957927

Citations 12

Authors

Yan-Ling Yin

Ting-Li Liu

Qian Yao

Yu-Xin Wang

Xue-Mei Wu

Xue-Ting Wang

Xin Yang

Jun-Ke Song

Guang-Hui Zhao

Affiliations

Soon will be listed here.

Abstract

Background: Cryptosporidium is an important zoonotic pathogen responsible for severe enteric diseases in humans and animals. However, the molecular mechanisms underlying host and Cryptosporidium interactions are still not clear.

Methods: To study the roles of circRNAs in host cells during Cryptosporidium infection, the expression profiles of circRNAs in HCT-8 cells infected with C. parvum were investigated using a microarray assay, and the regulatory role of a significantly upregulated circRNA, ciRS-7, was investigated during C. parvum infection.

Results: C. parvum infection caused notable alterations in the expression profiles of circRNAs in HCT-8 cells, and a total of 178 (including 128 up- and 50 downregulated) circRNAs were significantly differentially expressed following C. parvum infection. Among them, ciRS-7 was significantly upregulated and regulated the NF-κB signaling pathway by sponging miR-1270 during C. parvum infection. Furthermore, the ciRS-7/miR-1270/relA axis markedly affected the propagation of C. parvum in HCT-8 cells.

Conclusions: Our results revealed that ciRS-7 would promote C. parvum propagation by regulating the miR-1270/relA axis and affecting the NF-κB pathway. To the best of our knowledge, this is the first study to investigate the role of circRNA during Cryptosporidium infection, and the findings provide a novel view for implementing control strategies against Cryptosporidium infection.

Citing Articles

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