Maturing Heart Muscle Cells: Mechanisms and Transcriptomic Insights

Overview

Journal Semin Cell Dev Biol

Specialties Cell Biology
Reproductive Medicine

Date 2021 May 6

PMID 33952430

Citations 13

Authors

Sean A Murphy

Elaine Zhelan Chen

Leslie Tung

Kenneth R Boheler

Chulan Kwon

Affiliations

Soon will be listed here.

Abstract

Cardiomyocyte (CM) maturation is the transformation of differentiated fetal CMs into adult CMs that involves changes in morphology, cell function and metabolism, and the transcriptome. This process is, however, incomplete and ultimately arrested in pluripotent stem cell-derived CMs (PSC-CMs) in culture, which hinders their broad biomedical application. For this reason, enormous efforts are currently being made with the goal of generating mature PSC-CMs. In this review, we summarize key aspects of maturation observed in native CMs and discuss recent findings on the factors and mechanisms that regulate the process. Particular emphasis is put on transcriptional regulation and single-cell RNA-sequencing analysis that has emerged as a key tool to study time-series gene regulation and to determine the maturation state. We then discuss different biomimetic strategies to enhance PSC-CM maturation and discuss their effects at the single cell transcriptomic and functional levels.

Citing Articles

Molecular Regulation of Cardiomyocyte Maturation.

Shewale B, Ebrahim T, Samal A, Dubois N Curr Cardiol Rep. 2025; 27(1):32.

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Advancing Human iPSC-Derived Cardiomyocyte Hypoxia Resistance for Cardiac Regenerative Therapies through a Systematic Assessment of In Vitro Conditioning.

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A transcriptional enhancer regulates cardiac maturation.

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Unsupervised analysis of whole transcriptome data from human pluripotent stem cells cardiac differentiation.

P Agostinho S, A Branco M, E S Nogueira D, Diogo M, Cabral J, N Fred A Sci Rep. 2024; 14(1):3110.

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