Covalent Functionalization of Decellularized Tissues Accelerates Endothelialization

Overview

Journal Bioact Mater

Specialty Biomedical Engineering

Date 2021 May 3

PMID 33937589

Citations 5

Authors

Eleonora Dal Sasso

Annj Zamuner

Andrea Filippi

Filippo Romanato

Tiziana Palmosi

Luca Vedovelli

Dario Gregori

Jose Luis Gomez Ribelles

Teresa Russo

Antonio Gloria

Laura Iop

Gino Gerosa

Monica Dettin

Affiliations

Soon will be listed here.

Abstract

In the field of tissue regeneration, the lack of a stable endothelial lining may affect the hemocompatibility of both synthetic and biological replacements. These drawbacks might be prevented by specific biomaterial functionalization to induce selective endothelial cell (EC) adhesion. Decellularized bovine pericardia and porcine aortas were selectively functionalized with a REDV tetrapeptide at 10 M and 10 M working concentrations. The scaffold-bound peptide was quantified and REDV potential EC adhesion enhancement was evaluated by static seeding of human umbilical vein ECs. The viable cells and MTS production were statistically higher in functionalized tissues than in control. Scaffold histoarchitecture, geometrical features, and mechanical properties were unaffected by peptide anchoring. The selective immobilization of REDV was effective in accelerating ECs adhesion while promoting proliferation in functionalized decellularized tissues intended for blood-contacting applications.

Citing Articles

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