Selective Grafting of Protease-Resistant Adhesive Peptides on Titanium Surfaces

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2022 Dec 23

PMID 36557865

Authors

Annj Zamuner

Antonella Pasquato

Ignazio Castagliuolo

Monica Dettin

Paola Brun

Affiliations

Soon will be listed here.

Abstract

In orthopedic, dental, and maxillofacial fields, joint prostheses, plates, and screws are widely used in the treatment of problems related to bone tissue. However, the use of these prosthetic systems is not free from complications: the fibrotic encapsulation of endosseous implants often prevents optimal integration of the prostheses with the surrounding bone. To overcome these issues, biomimetic titanium implants have been developed where synthetic peptides have been selectively grafted on titanium surfaces via Schiff base formation. We used the retro-inverted sequence (DHVPX) from [351-359] human Vitronectin and its dimer (D2HVP). Both protease-resistant peptides showed increased human osteoblast adhesion and proliferation, an augmented number of focal adhesions, and cellular spreading with respect to the control. D2HVP-grafted samples significantly enhance Secreted Phosphoprotein 1, Integrin Binding Sialoprotein, and Vitronectin gene expression vs. control. An estimation of peptide surface density was determined by Two-photon microscopy analysis on a silanized glass model surface labeled with a fluorescent analog.

Citing Articles

Proteolytically Resistant Bioactive Peptide-Grafted Sr/Mg-Doped Hardystonite Foams: Comparison of Two Covalent Functionalization Strategies.

Zamuner A, Zeni E, Elsayed H, Di Foggia M, Taddei P, Pasquato A Biomimetics (Basel). 2023; 8(2).

PMID: 37218771 PMC: 10204510. DOI: 10.3390/biomimetics8020185.

References

Ruoslahti E . RGD and other recognition sequences for integrins. Annu Rev Cell Dev Biol. 1996; 12:697-715. DOI: 10.1146/annurev.cellbio.12.1.697. View

Smeets R, Stadlinger B, Schwarz F, Beck-Broichsitter B, Jung O, Precht C . Impact of Dental Implant Surface Modifications on Osseointegration. Biomed Res Int. 2016; 2016:6285620. PMC: 4958483. DOI: 10.1155/2016/6285620. View

Brun P, Scorzeto M, Vassanelli S, Castagliuolo I, Palu G, Ghezzo F . Mechanisms underlying the attachment and spreading of human osteoblasts: from transient interactions to focal adhesions on vitronectin-grafted bioactive surfaces. Acta Biomater. 2012; 9(4):6105-15. DOI: 10.1016/j.actbio.2012.12.018. View

Wittneben J, Millen C, Bragger U . Clinical performance of screw- versus cement-retained fixed implant-supported reconstructions--a systematic review. Int J Oral Maxillofac Implants. 2014; 29 Suppl:84-98. DOI: 10.11607/jomi.2014suppl.g2.1. View

Sawyer A, Hennessy K, Bellis S . The effect of adsorbed serum proteins, RGD and proteoglycan-binding peptides on the adhesion of mesenchymal stem cells to hydroxyapatite. Biomaterials. 2006; 28(3):383-92. DOI: 10.1016/j.biomaterials.2006.08.031. View

Chen Q, Zhang D, Gu J, Zhang H, Wu X, Cao C . The impact of antifouling layers in fabricating bioactive surfaces. Acta Biomater. 2021; 126:45-62. DOI: 10.1016/j.actbio.2021.03.022. View

Jurczak P, Witkowska J, Rodziewicz-Motowidlo S, Lach S . Proteins, peptides and peptidomimetics as active agents in implant surface functionalization. Adv Colloid Interface Sci. 2019; 276:102083. DOI: 10.1016/j.cis.2019.102083. View

Zamuner A, Brun P, Scorzeto M, Sica G, Castagliuolo I, Dettin M . Smart biomaterials: Surfaces functionalized with proteolytically stable osteoblast-adhesive peptides. Bioact Mater. 2018; 2(3):121-130. PMC: 5935176. DOI: 10.1016/j.bioactmat.2017.05.004. View

Fetse J, Zhao Z, Liu H, Mamani U, Mustafa B, Adhikary P . Discovery of Cyclic Peptide Inhibitors Targeting PD-L1 for Cancer Immunotherapy. J Med Chem. 2022; 65(18):12002-12013. PMC: 10671706. DOI: 10.1021/acs.jmedchem.2c00539. View

10.

Srinivasan M, Meyer S, Mombelli A, Muller F . Dental implants in the elderly population: a systematic review and meta-analysis. Clin Oral Implants Res. 2016; 28(8):920-930. DOI: 10.1111/clr.12898. View

11.

Mavrogenis A, Dimitriou R, Parvizi J, Babis G . Biology of implant osseointegration. J Musculoskelet Neuronal Interact. 2009; 9(2):61-71. View

12.

Yamada K . Adhesive recognition sequences. J Biol Chem. 1991; 266(20):12809-12. View

13.

Rupp F, Liang L, Geis-Gerstorfer J, Scheideler L, Huttig F . Surface characteristics of dental implants: A review. Dent Mater. 2017; 34(1):40-57. DOI: 10.1016/j.dental.2017.09.007. View

14.

Yang B, Zhou X, Yu H, Wu Y, Bao C, Man Y . [Advances in titanium dental implant surface modification]. Hua Xi Kou Qiang Yi Xue Za Zhi. 2019; 37(2):124-129. PMC: 7030153. DOI: 10.7518/hxkq.2019.02.002. View

15.

Cacchioli A, Ravanetti F, Bagno A, Dettin M, Gabbi C . Human Vitronectin-Derived Peptide Covalently Grafted onto Titanium Surface Improves Osteogenic Activity: A Pilot In Vivo Study on Rabbits. Tissue Eng Part A. 2009; 15(10):2917-26. DOI: 10.1089/ten.TEA.2008.0542. View

16.

Ede N, Eagle S, Wickham G, Bray A, Warne B, Shoemaker K . Solid phase synthesis of peptide aldehyde protease inhibitors. Probing the proteolytic sites of hepatitis C virus polyprotein. J Pept Sci. 2000; 6(1):11-8. DOI: 10.1002/(SICI)1099-1387(200001)6:1<11::AID-PSC229>3.0.CO;2-#. View

17.

Goriainov V, Cook R, Latham J, Dunlop D, Oreffo R . Bone and metal: an orthopaedic perspective on osseointegration of metals. Acta Biomater. 2014; 10(10):4043-57. DOI: 10.1016/j.actbio.2014.06.004. View

18.

Battista E, Causa F, Lettera V, Panzetta V, Guarnieri D, Fusco S . Ligand engagement on material surfaces is discriminated by cell mechanosensoring. Biomaterials. 2015; 45:72-80. DOI: 10.1016/j.biomaterials.2014.12.012. View

19.

Dettin M, Conconi M, Gambaretto R, Bagno A, Di Bello C, Menti A . Effect of synthetic peptides on osteoblast adhesion. Biomaterials. 2005; 26(22):4507-15. DOI: 10.1016/j.biomaterials.2004.11.023. View

20.

Mantripragada V, Lecka-Czernik B, Ebraheim N, Jayasuriya A . An overview of recent advances in designing orthopedic and craniofacial implants. J Biomed Mater Res A. 2013; 101(11):3349-64. PMC: 4854641. DOI: 10.1002/jbm.a.34605. View