New Hyaluronic Acid/Polyethylene Oxide-Based Electrospun Nanofibers: Design, Characterization and In Vitro Biological Evaluation

Overview

Journal Polymers (Basel)

Publisher MDPI

Date 2021 Apr 30

PMID 33920998

Citations 8

Authors

Oana Maria Ionescu

Arn Mignon

Andreea Teodora Iacob

Natalia Simionescu

Luminita Georgeta Confederat

Cristina Tuchilus

Lenuta Profire

Affiliations

Soon will be listed here.

Abstract

Natural compounds have been used as wound-healing promoters and are also present in today's clinical proceedings. In this research, different natural active components such as propolis, Manuka honey, insulin, L-arginine, and infusion were included into hyaluronic acid/poly(ethylene)oxide-based electrospun nanofiber membranes to design innovative wound-dressing biomaterials. Morphology and average fiber diameter were analyzed by scanning electron microscopy. Chemical composition was proved by Fourier transform infrared spectroscopy, which indicated successful incorporation of the active components. The nanofiber membranes with propolis and showed best antioxidant activity, cytocompatibility, and antimicrobial properties against pathogen strains and and had an average diameter of 217 ± 19 nm with smooth surface aspect. Water vapor transmission rate was in agreement with the range suitable for preventing infections or wound dehydration (~5000 g/m 24 h). Therefore, the developed hyaluronic acid/poly(ethylene)oxide nanofibers with additional natural components showed favorable features for clinical use as wound dressings.

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References

Hussein Y, El-Fakharany E, Kamoun E, Loutfy S, Amin R, Taha T . Electrospun PVA/hyaluronic acid/L-arginine nanofibers for wound healing applications: Nanofibers optimization and in vitro bioevaluation. Int J Biol Macromol. 2020; 164:667-676. DOI: 10.1016/j.ijbiomac.2020.07.126. View

Ruckriemen J, Klemm O, Henle T . Manuka honey (Leptospermum scoparium) inhibits jack bean urease activity due to methylglyoxal and dihydroxyacetone. Food Chem. 2017; 230:540-546. DOI: 10.1016/j.foodchem.2017.03.075. View

Oryan A, Alemzadeh E . Effects of insulin on wound healing: A review of animal and human evidences. Life Sci. 2017; 174:59-67. DOI: 10.1016/j.lfs.2017.02.015. View

Halstead F, Rauf M, Bamford A, Wearn C, Bishop J, Burt R . Antimicrobial dressings: Comparison of the ability of a panel of dressings to prevent biofilm formation by key burn wound pathogens. Burns. 2015; 41(8):1683-1694. DOI: 10.1016/j.burns.2015.06.005. View

Ahire J, Robertson D, van Reenen A, Dicks L . Polyethylene oxide (PEO)-hyaluronic acid (HA) nanofibers with kanamycin inhibits the growth of Listeria monocytogenes. Biomed Pharmacother. 2016; 86:143-148. DOI: 10.1016/j.biopha.2016.12.006. View

Seon-Lutz M, Couffin A, Vignoud S, Schlatter G, Hebraud A . Electrospinning in water and in situ crosslinking of hyaluronic acid / cyclodextrin nanofibers: Towards wound dressing with controlled drug release. Carbohydr Polym. 2019; 207:276-287. DOI: 10.1016/j.carbpol.2018.11.085. View

Weber M, Steinle H, Golombek S, Hann L, Schlensak C, Wendel H . Blood-Contacting Biomaterials: Evaluation of the Hemocompatibility. Front Bioeng Biotechnol. 2018; 6:99. PMC: 6054932. DOI: 10.3389/fbioe.2018.00099. View

Debats I, Wolfs T, Gotoh T, Cleutjens J, Peutz-Kootstra C, van der Hulst R . Role of arginine in superficial wound healing in man. Nitric Oxide. 2009; 21(3-4):175-83. DOI: 10.1016/j.niox.2009.07.006. View

Zhao X, Wu H, Guo B, Dong R, Qiu Y, Ma P . Antibacterial anti-oxidant electroactive injectable hydrogel as self-healing wound dressing with hemostasis and adhesiveness for cutaneous wound healing. Biomaterials. 2017; 122:34-47. DOI: 10.1016/j.biomaterials.2017.01.011. View

10.

Pedram Rad Z, Mokhtari J, Abbasi M . Calendula officinalis extract/PCL/Zein/Gum arabic nanofibrous bio-composite scaffolds via suspension, two-nozzle and multilayer electrospinning for skin tissue engineering. Int J Biol Macromol. 2019; 135:530-543. DOI: 10.1016/j.ijbiomac.2019.05.204. View

11.

Motealleh B, Zahedi P, Rezaeian I, Moghimi M, Abdolghaffari A, Zarandi M . Morphology, drug release, antibacterial, cell proliferation, and histology studies of chamomile-loaded wound dressing mats based on electrospun nanofibrous poly(ɛ-caprolactone)/polystyrene blends. J Biomed Mater Res B Appl Biomater. 2013; 102(5):977-87. DOI: 10.1002/jbm.b.33078. View

12.

Emanuelli T, Burgeiro A, Carvalho E . Effects of insulin on the skin: possible healing benefits for diabetic foot ulcers. Arch Dermatol Res. 2016; 308(10):677-694. DOI: 10.1007/s00403-016-1686-z. View

13.

Aduba D, Yang H . Polysaccharide Fabrication Platforms and Biocompatibility Assessment as Candidate Wound Dressing Materials. Bioengineering (Basel). 2017; 4(1). PMC: 5590441. DOI: 10.3390/bioengineering4010001. View

14.

Hossen M, Ali M, Jahurul M, Abdel-Daim M, Gan S, Khalil M . Beneficial roles of honey polyphenols against some human degenerative diseases: A review. Pharmacol Rep. 2017; 69(6):1194-1205. DOI: 10.1016/j.pharep.2017.07.002. View

15.

Adeli H, Khorasani M, Parvazinia M . Wound dressing based on electrospun PVA/chitosan/starch nanofibrous mats: Fabrication, antibacterial and cytocompatibility evaluation and in vitro healing assay. Int J Biol Macromol. 2018; 122:238-254. DOI: 10.1016/j.ijbiomac.2018.10.115. View

16.

Ajmal G, Bonde G, Mittal P, Khan G, Pandey V, Bakade B . Biomimetic PCL-gelatin based nanofibers loaded with ciprofloxacin hydrochloride and quercetin: A potential antibacterial and anti-oxidant dressing material for accelerated healing of a full thickness wound. Int J Pharm. 2019; 567:118480. DOI: 10.1016/j.ijpharm.2019.118480. View

17.

Bhardwaj N, Kundu S . Electrospinning: a fascinating fiber fabrication technique. Biotechnol Adv. 2010; 28(3):325-47. DOI: 10.1016/j.biotechadv.2010.01.004. View

18.

Tang Y, Lan X, Liang C, Zhong Z, Xie R, Zhou Y . Honey loaded alginate/PVA nanofibrous membrane as potential bioactive wound dressing. Carbohydr Polym. 2019; 219:113-120. DOI: 10.1016/j.carbpol.2019.05.004. View

19.

Boateng J, Catanzano O . Advanced Therapeutic Dressings for Effective Wound Healing--A Review. J Pharm Sci. 2015; 104(11):3653-3680. DOI: 10.1002/jps.24610. View

20.

Hrynyk M, Martins-Green M, Barron A, Neufeld R . Sustained prolonged topical delivery of bioactive human insulin for potential treatment of cutaneous wounds. Int J Pharm. 2010; 398(1-2):146-54. DOI: 10.1016/j.ijpharm.2010.07.052. View