Immunomodulatory Activities of L. Leaf Juice in a Non-Lethal, Symptomatic Dengue Mouse Model

Overview

Journal Pathogens

Date 2021 Apr 30

PMID 33919457

Citations 11

Authors

Mohd Ridzuan Mohd Abd Razak

Nor Azrina Norahmad

Nur Hana Md Jelas

Adlin Afzan

Norazlan Mohmad Misnan

Adiratna Mat Ripen

Ravindran Thayan

Murizal Zainol

Ami Fazlin Syed Mohamed

Affiliations

Soon will be listed here.

Abstract

The role of L. leaf juice in immune dysregulation caused by dengue virus infection remains unclear. This study aimed to investigate the immunomodulatory activities of the freeze-dried leaf juice (FCPLJ) on AG129 mice infected with a clinical DENV-2 (DMOF015) isolate. The infected AG129 mice were orally treated with 500 and 1000 mg/kg/day of FCPLJ, for three days. Platelet, leukocyte, lymphocyte and neutrophil counts were microscopically determined. The level of plasma proinflammatory cytokines was measured by multiplex immunoassay. The levels of intracellular cytokines and viral RNA were determined by RT-qPCR technique. The results showed that the FCPLJ treatment increased the total white blood cell and neutrophil counts in the infected mice. The FCPLJ treatment decreased the level of GM-CSF, GRO-alpha, IL-1 beta, IL-6, MCP-1 and MIP-1 beta in the plasma of the infected mice. The intracellular IL-6 and viral RNA levels in the liver of infected mice were decreased by the FCPLJ treatment. In conclusion, this study supports the potential immunomodulatory role of the FCPLJ in a non-lethal, symptomatic dengue mouse model. Further studies on the action mechanism of the leaf juice and its possible use as adjunctive dengue immunotherapy are warranted.

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