Engineered NS1 for Sensitive, Specific Zika Virus Diagnosis from Patient Serology

Overview

Journal Emerg Infect Dis

Specialty Infectious Diseases

Date 2021 Apr 26

PMID 33900180

Citations 3

Authors

Thai Leong Yap

Shin Yee Hong

Jun Hui Soh

Lekha Ravichandraprabhu

Vanessa W X Lim

Hsi-Min Chan

Tommy Z X Ong

Ying Ping Chua

Shi En Koh

Huajing Wang

Yee Sin Leo

Jackie Y Ying

William Sun

Affiliations

Soon will be listed here.

Abstract

Dengue virus (DENV) and Zika virus (ZIKV) belong to the Flaviviridae family of viruses spread by Aedes aegypti mosquitoes in tropical and subtropical areas. Accurate diagnostic tests to differentiate the 2 infections are necessary for patient management and disease control. Using characterized ZIKV and DENV patient plasma in a blind manner, we validated an ELISA and a rapid immunochromatographic test for ZIKV detection. We engineered the ZIKV nonstructural protein 1 (NS1) for sensitive serologic detection with low cross reactivity against dengue and developed monoclonal antibodies specific for the ZIKV NS1 antigen. As expected, the serologic assays performed better with convalescent than acute plasma samples; the sensitivity ranged from 71% to 88%, depending on the performance of individual tests (IgM/IgG/NS1). Although serologic tests were generally less sensitive with acute samples, our ZIKV NS1 antibodies were able to complement the serologic tests to achieve greater sensitivity for detecting early infections.

Citing Articles

Use of Envelope Domain III Protein for the Detection of IgG Type Antibodies Specific to Zika Virus by Indirect ELISA.

Ndiaye O, Diagne C, Abd El Wahed A, Dia F, Dia M, Faye A Diagnostics (Basel). 2023; 13(3).

PMID: 36766567 PMC: 9913938. DOI: 10.3390/diagnostics13030462.

DNA aptamer selection and construction of an aptasensor based on graphene FETs for Zika virus NS1 protein detection.

Almeida N, Sousa T, Santos V, Lacerda C, Silva T, Grenfell R Beilstein J Nanotechnol. 2022; 13:873-881.

PMID: 36105684 PMC: 9443353. DOI: 10.3762/bjnano.13.78.

Competitive ELISA for a serologic test to detect dengue serotype-specific anti-NS1 IgGs using high-affinity UB-DNA aptamers.

Matsunaga K, Kimoto M, Lim V, Thein T, Vasoo S, Leo Y Sci Rep. 2021; 11(1):18000.

PMID: 34504185 PMC: 8429655. DOI: 10.1038/s41598-021-97339-8.

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