Polysaccharide Dual Coating of Yeast Capsules for Stabilization of Anthocyanins

The dual coated yeast capsules for anthocyanin encapsulation and stabilization were fabricated. Anthocyanins were preloaded in hollow yeast capsules, and then the dual coating was performed by deposition of opposite charged polysaccharides using layer-by-layer technique. The combination of positively charged chitosan and negatively charged chondroitin sulfate was found to confer the yeast capsules with the highest encapsulation efficiency and retention rate of anthocyanins. Additionally, the coated yeast capsules featured high tolerance to environmental stresses (i.e., oxygen, ascorbic acid, and heat) and therefore effectively inhibited the degradation of anthocyanins. These stabilizing effects were related to the formation of high penetration barrier provided by the double layers of polysaccharides, as well as the enhanced hydrophobic microenvironment in the capsules. Further development of the polysaccharide-coated yeast capsules may hold promise for the controlled delivery of other water-soluble bioactive components.