Glycine Regulates Mucosal Immunity and the Intestinal Microbial Composition in Weaned Piglets

Overview

Journal Amino Acids

Specialty Biochemistry

Date 2021 Apr 11

PMID 33839961

Citations 20

Authors

Yun Ji

Xiaoxiao Fan

Yunchang Zhang

Ju Li

Zhaolai Dai

Zhenlong Wu

Affiliations

Soon will be listed here.

Abstract

Glycine is an amino acid with a diverse array of health benefits regarding metabolism, immunity, and development. The aim of this study was to test the hypothesis that glycine supplementation alters the intestinal microbial composition and improves the intestinal mucosal immunity of weaned piglets. One hundred and twenty-eight weaned piglets divided into 4 groups were fed with a corn- and soybean meal-based diet supplemented with 0 (control), 0.5, 1, or 2% glycine for 7 days. The intestinal microbiota and tissue samples from the control and the 2% glycine-supplemented piglets were collected for determination of the composition of microbial community and the intestinal mucosal barrier function. Piglets fed with diet containing 2% glycine, instead of 0.5% or 1% glycine, presented elevated average daily gain and feed conversion ratio, as compared with the control. 2% glycine enhanced the abundance of mucins in the jejunum and ileum and mRNA level of porcine β-defensin (pBD) 2 and pBD-3, as well as the protein level of secretory immunoglobulin A (sIgA) in the jejunum. The mRNA expression of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6, and the protein level of phosphorylated p38 mitogen-activated protein kinase (MAPK), signal transducer and activator of transcription 3 (STAT3), nuclear factor (NF)-κB p65, and claudin-2 in the jejunum were lower in the 2% glycine group than that in the control. In addition, an elevated ratio of CD4+/CD8+ T lymphocytes was observed in the jejunum of piglets receiving diet supplemented with 2% glycine. The colon content of piglets fed with 2% glycine exhibited a reduction in abundance of pathogenic bacteria (Escherichia-Shigella, Clostridium, and Burkholderiales) and an increase in short-chain fatty acid-producing bacteria (Blautia, Lachnospiraceae, Anaerostipes, and Prevotella) in comparison with the control. We conclude that dietary supplementation with 2% glycine improves the intestinal immunological barrier function and the microbial composition, therefore, contributing to the growth performance of weaned piglets.

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References

Adeola O, King D . Developmental changes in morphometry of the small intestine and jejunal sucrase activity during the first nine weeks of postnatal growth in pigs. J Anim Sci. 2005; 84(1):112-8. DOI: 10.2527/2006.841112x. View

Al-Sadi R, Ye D, Boivin M, Guo S, Hashimi M, Ereifej L . Interleukin-6 modulation of intestinal epithelial tight junction permeability is mediated by JNK pathway activation of claudin-2 gene. PLoS One. 2014; 9(3):e85345. PMC: 3963839. DOI: 10.1371/journal.pone.0085345. View

Santana L, Inada A, Santo B, Filiu W, Pott A, Alves F . Nutraceutical Potential of in Metabolic Syndrome. Nutrients. 2019; 11(7). PMC: 6682863. DOI: 10.3390/nu11071608. View

Ananthakrishnan A, Luo C, Yajnik V, Khalili H, Garber J, Stevens B . Gut Microbiome Function Predicts Response to Anti-integrin Biologic Therapy in Inflammatory Bowel Diseases. Cell Host Microbe. 2017; 21(5):603-610.e3. PMC: 5705050. DOI: 10.1016/j.chom.2017.04.010. View

Atay O, Skotheim J . Spatial and temporal signal processing and decision making by MAPK pathways. J Cell Biol. 2017; 216(2):317-330. PMC: 5294789. DOI: 10.1083/jcb.201609124. View

Walters K, Zhu R, Welge M, Scherler K, Park J, Rahil Z . Differential Effects of Influenza Virus NA, HA Head, and HA Stalk Antibodies on Peripheral Blood Leukocyte Gene Expression during Human Infection. mBio. 2019; 10(3). PMC: 6520452. DOI: 10.1128/mBio.00760-19. View

Bien J, Palagani V, Bozko P . The intestinal microbiota dysbiosis and Clostridium difficile infection: is there a relationship with inflammatory bowel disease?. Therap Adv Gastroenterol. 2013; 6(1):53-68. PMC: 3539291. DOI: 10.1177/1756283X12454590. View

DAcquisto F, Crompton T . CD3+CD4-CD8- (double negative) T cells: saviours or villains of the immune response?. Biochem Pharmacol. 2011; 82(4):333-40. DOI: 10.1016/j.bcp.2011.05.019. View

Dupont A, Heinbockel L, Brandenburg K, Hornef M . Antimicrobial peptides and the enteric mucus layer act in concert to protect the intestinal mucosa. Gut Microbes. 2014; 5(6):761-5. PMC: 4615892. DOI: 10.4161/19490976.2014.972238. View

10.

Fan X, Li S, Wu Z, Dai Z, Li J, Wang X . Glycine supplementation to breast-fed piglets attenuates post-weaning jejunal epithelial apoptosis: a functional role of CHOP signaling. Amino Acids. 2018; 51(3):463-473. DOI: 10.1007/s00726-018-2681-9. View

11.

Fuchs S, Peeters-Scholte C, de Barse M, Roeleveld M, Klomp L, Berger R . Increased concentrations of both NMDA receptor co-agonists D-serine and glycine in global ischemia: a potential novel treatment target for perinatal asphyxia. Amino Acids. 2011; 43(1):355-63. PMC: 3374112. DOI: 10.1007/s00726-011-1086-9. View

12.

Grotz M, Pape H, van Griensven M, Stalp M, Rohde F, Bock D . Glycine reduces the inflammatory response and organ damage in a two-hit sepsis model in rats. Shock. 2001; 16(2):116-21. DOI: 10.1097/00024382-200116020-00006. View

13.

Hartog A, Leenders I, van der Kraan P, Garssen J . Anti-inflammatory effects of orally ingested lactoferrin and glycine in different zymosan-induced inflammation models: evidence for synergistic activity. Int Immunopharmacol. 2007; 7(13):1784-92. DOI: 10.1016/j.intimp.2007.09.019. View

14.

Hu C, Xiao K, Luan Z, Song J . Early weaning increases intestinal permeability, alters expression of cytokine and tight junction proteins, and activates mitogen-activated protein kinases in pigs. J Anim Sci. 2012; 91(3):1094-101. DOI: 10.2527/jas.2012-5796. View

15.

Hussain T, Kulshreshtha K, Yadav V, Katoch K . CD4+, CD8+, CD3+ cell counts and CD4+/CD8+ ratio among patients with mycobacterial diseases (leprosy, tuberculosis), HIV infections, and normal healthy adults: a comparative analysis of studies in different regions of India. J Immunoassay Immunochem. 2014; 36(4):420-43. DOI: 10.1080/15321819.2014.978082. View

16.

Johansson M, Hansson G . Immunological aspects of intestinal mucus and mucins. Nat Rev Immunol. 2016; 16(10):639-49. PMC: 6435297. DOI: 10.1038/nri.2016.88. View

17.

Le N, Mazahery C, Nguyen K, Levine A . Regulation of Intestinal Epithelial Barrier and Immune Function by Activated T Cells. Cell Mol Gastroenterol Hepatol. 2020; 11(1):55-76. PMC: 7596298. DOI: 10.1016/j.jcmgh.2020.07.004. View

18.

Li X, Bradford B, Wheeler M, Stimpson S, Pink H, Brodie T . Dietary glycine prevents peptidoglycan polysaccharide-induced reactive arthritis in the rat: role for glycine-gated chloride channel. Infect Immun. 2001; 69(9):5883-91. PMC: 98707. DOI: 10.1128/IAI.69.9.5883-5891.2001. View

19.

Li W, Sun K, Ji Y, Wu Z, Wang W, Dai Z . Glycine Regulates Expression and Distribution of Claudin-7 and ZO-3 Proteins in Intestinal Porcine Epithelial Cells. J Nutr. 2016; 146(5):964-9. DOI: 10.3945/jn.115.228312. View

20.

Lopez-Pedrosa J, Torres M, Fernandez M, Rios A, Gil A . Severe malnutrition alters lipid composition and fatty acid profile of small intestine in newborn piglets. J Nutr. 1998; 128(2):224-33. DOI: 10.1093/jn/128.2.224. View