Hexane Extract of Increases Adipocyte Differentiation by Acting on Wnt/β-Catenin Signaling Pathway

Overview

Journal Life (Basel)

Specialty Biology

Date 2021 Apr 3

PMID 33804020

Authors

Ui Jeong Yun

Chu Won Nho

Kye Won Park

Dong Kwon Yang

Affiliations

Soon will be listed here.

Abstract

has been heavily investigated for the treatment of various diseases. This paper attempts to show that can modulate adipocyte differentiation of preadipocytes. To establish this, we investigated the effects of extract in peroxisome proliferator-activated receptor γ (PPARγ) expression, adipogenesis, and the underlying molecular mechanisms in C3H10T1/2 and 3T3-L1 cells. Our data showed that methanol extract increased lipid accumulation and promoted adipocyte differentiation. Further studies on the fractionation with various solvents led to the identification of hexane extract (CJHE) as the most potent inducer of adipocyte differentiation. CJHE consistently increased lipid accumulation and adipocyte marker expression including Pparγ and it acted during the early stages of adipocyte differentiation. Mechanistic studies revealed that CJHE and a Wnt inhibitor similarly stimulated adipogenesis and were active in Wnt-selective reporter assays. The effects of CJHE were inhibited by Wnt3a protein treatment and were significantly blunted in β-catenin-silenced cells, further suggesting that CJHE acted on Wnt pathways to promote adipogenesis. We also showed that extracts generated from different plant parts similarly promoted adipocyte differentiation. These results identified as a pro-adipogenic natural product and suggest its potential use in metabolic syndrome.

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