Euodia Sutchuenensis Dode Extract Stimulates Osteoblast Differentiation Via Wnt/β-catenin Pathway Activation

Overview

Journal Exp Mol Med

Specialties Biochemistry
Molecular Biology

Date 2015 Mar 21

PMID 25792220

Citations 12

Authors

Jeong-Ha Hwang

Pu-Hyeon Cha

Gyoonhee Han

Tran The Bach

Do Sik Min

Kang-Yell Choi

Affiliations

Soon will be listed here.

Abstract

The Wnt/β-catenin pathway has a role in osteoblast differentiation and bone formation. We screened 100 plant extracts and identified an extract from Euodia sutchuenensis Dode (ESD) leaf and young branch as an effective activator of the Wnt/β-catenin pathway. ESD extract increased β-catenin levels and β-catenin nuclear accumulation in murine primary osteoblasts. The ESD extract also increased mRNA levels of osteoblast markers, including RUNX2, BMP2 and COL1A1, and enhanced alkaline phosphatase (ALP) activity in murine primary osteoblasts. Both ESD extract-induced β-catenin increment and ALP activation were abolished by β-catenin knockdown, confirming that the Wnt/β-catenin pathway functions in osteoblast differentiation. ESD extract enhanced terminal osteoblast differentiation as shown by staining with Alizarin Red S and significantly increased murine calvarial bone thickness. This study shows that ESD extract stimulates osteoblast differentiation via the Wnt/β-catenin pathway and enhances murine calvarial bone formation ex vivo.

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