Taxonomic Identification of Different Species of the Genus Aeromonas by Whole-Genome Sequencing and Use of Their Species-Specific β-Lactamases As Phylogenetic Markers

Overview

Journal Antibiotics (Basel)

Specialty Pharmacology

Date 2021 Apr 3

PMID 33800590

Citations 7

Authors

Xavier Bertran

Marc Rubio

Laura Gomez

Teresa Llovet

Carme Munoz

Ferran Navarro

Elisenda Miro

Affiliations

Soon will be listed here.

Abstract

Some species, potentially pathogenic for humans, are known to express up to three different classes of chromosomal β-lactamases, which may become hyperproduced and cause treatment failure. The aim of this study was to assess the utility of these species-specific β-lactamase genes as phylogenetic markers using whole-genome sequencing data. Core-genome alignments were generated for 36 genomes from seven different species and scanned for antimicrobial resistance genes. Core-genome alignment confirmed the MALDI-TOF identification of most of the isolates and re-identified an isolate as . Three (B, C and D) of the four Ambler classes of β-lactamase genes were found in and ( and ). only showed class-B- and class-D-like matches ( and ), whereas those for and were class C and D (, and ). The phylogenetic tree derived from concatenated sequences of β-lactamase genes successfully clustered each species. Some isolates also had resistance to sulfonamides, quinolones and aminoglycosides. Whole-genome sequencing proved to be a useful method to identify at the species level, which led to the unexpected identification of and . and revealed the resistome of each isolate.

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