Evaluation of the MALDI-TOF MS Profiling for Identification of Newly Described Aeromonas Spp

Overview

Journal Folia Microbiol (Praha)

Publisher Springer

Specialty Microbiology

Date 2014 Dec 19

PMID 25520239

Citations 11

Authors

Andrea Vavrova

Tereza Balazova

Ivo Sedlacek

Ludmila Tvrzova

Ondrej Sedo

Affiliations

Soon will be listed here.

Abstract

The genus Aeromonas comprises primarily aquatic bacteria and also serious human and animal pathogens with the occurrence in clinical material, drinking water, and food. Aeromonads are typical for their complex taxonomy and nomenclature and for limited possibilities of identification to the species level. According to studies describing the use of MALDI-TOF MS in diagnostics of aeromonads, this modern chemotaxonomical approach reveals quite high percentage of correctly identified isolates. We analyzed 64 Aeromonas reference strains from the set of 27 species. After extending the range of analyzed Aeromonas species by newly described ones, we proved that MALDI-TOF MS procedure accompanied by Biotyper tool is not a reliable diagnostic technique for aeromonads. We obtained quite high percentage of false-positive, incorrect, and uncertain results. The identification of newly described species is accompanied with misidentifications that were observed also in the case of pathogenic aeromonads.

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