Subunits of the PBAP Chromatin Remodeler Are Capable of Mediating Enhancer-Driven Transcription in

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2021 Apr 3

PMID 33799739

Citations 7

Authors

Yulii V Shidlovskii

Oleg V Bylino

Alexander V Shaposhnikov

Zaur M Kachaev

Lyubov A Lebedeva

Valeria V Kolesnik

Diego Amendola

Giovanna De Simone

Nadia Formicola

Paul Schedl

Filomena Anna Digilio

Ennio Giordano

Affiliations

Soon will be listed here.

Abstract

The chromatin remodeler SWI/SNF is an important participant in gene activation, functioning predominantly by opening the chromatin structure on promoters and enhancers. Here, we describe its novel mode of action in which SWI/SNF factors mediate the targeted action of an enhancer. We studied the functions of two signature subunits of PBAP subfamily, BAP170 and SAYP, in . These subunits were stably tethered to a transgene reporter carrying the core promoter. The tethered subunits mediate transcription of the reporter in a pattern that is generated by enhancers close to the insertion site in multiple loci throughout the genome. Both tethered SAYP and BAP170 recruit the whole PBAP complex to the reporter promoter. However, we found that BAP170-dependent transcription is more resistant to the depletion of other PBAP subunits, suggesting that BAP170 may play a more critical role in establishing enhancer-dependent transcription.

Citing Articles

Subunits Med12 and Med13 of Mediator Cooperate with Subunits SAYP and Bap170 of SWI/SNF in Active Transcription in .

Shidlovskii Y, Ulianova Y, Shaposhnikov A, Kolesnik V, Pravednikova A, Stepanov N Int J Mol Sci. 2024; 25(23).

PMID: 39684492 PMC: 11641163. DOI: 10.3390/ijms252312781.

Effects of Overexpression of Specific Subunits SAYP, BAP170 of the Chromatin Remodeling Complex in Drosophila Melanogaster.

Chmykhalo V, Shidlovskii Y, Lebedeva L, Schedl P, Giordano E Dokl Biochem Biophys. 2024; 519(1):588-592.

PMID: 39480634 DOI: 10.1134/S160767292460088X.

PHD finger protein 10 promotes cell proliferation by regulating CD44 transcription in gastric cancer.

Fan Z, Jiang X, Yan W, Li J, Yan M, Liu B Heliyon. 2024; 10(7):e29109.

PMID: 38601625 PMC: 11004891. DOI: 10.1016/j.heliyon.2024.e29109.

The Drosophila BEAF insulator protein interacts with the polybromo subunit of the PBAP chromatin remodeling complex.

McKowen J, Avva S, Maharjan M, Duarte F, Tome J, Judd J G3 (Bethesda). 2022; 12(11).

PMID: 36029240 PMC: 9635645. DOI: 10.1093/g3journal/jkac223.

Application of the 3C Method to Study the Developmental Genes in Larvae.

Bylino O, Ibragimov A, Digilio F, Giordano E, Shidlovskii Y Front Genet. 2022; 13:734208.

PMID: 35910225 PMC: 9335292. DOI: 10.3389/fgene.2022.734208.

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