Live Intravital Intestine with Blood Flow Visualization in Neonatal Mice Using Two-photon Laser Scanning Microscopy

Overview

Journal Bio Protoc

Specialty Biology

Date 2021 Apr 2

PMID 33796611

Citations 4

Authors

Yuhki Koike

Bo Li

Yong Chen

Niloofar Ganji

Mashriq Alganabi

Hiromu Miyake

Carol Lee

Alison Hock

Richard Wu

Keiichi Uchida

Mikihiro Inoue

Paul Delgado-Olguin

Agostino Pierro

Affiliations

Soon will be listed here.

Abstract

This protocol describes a novel technique to investigate the microcirculation dynamics underlying the pathology in the small intestine of neonatal mice using two-photon laser-scanning microscopy (TPLSM). Recent technological advances in multi-photon microscopy allow intravital analysis of different organs such as the liver, brain and intestine. Despite these advances, live visualization and analysis of the small intestine in neonatal rodents remain technically challenging. We herein provide a detailed description of a novel method to capture high resolution and stable images of the small intestine in neonatal mice as early as postnatal day 0. This imaging technique allows a comprehensive understanding of the development and blood flow dynamics in small intestine microcirculation.

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