HYOU1 Facilitates Proliferation, Invasion and Glycolysis of Papillary Thyroid Cancer Via Stabilizing LDHB MRNA

Overview

Journal J Cell Mol Med

Specialties Cell Biology
Molecular Biology

Date 2021 Apr 1

PMID 33792181

Citations 14

Authors

Jia-Mei Wang

Jing-Yi Jiang

Da-Lin Zhang

Xin Du

Tong Wu

Zhen-Xian Du

Affiliations

Soon will be listed here.

Abstract

HYOU1 is upregulated in many kinds of cancer cells, and its high expression is associated with tumour invasiveness and poor prognosis. However, the role of HYOU1 in papillary thyroid cancer (PTC) development and progression remains to be elucidated. Here, we reported that HYOU1 was highly expressed in human PTC and associated with poor prognosis. HYOU1 silencing suppressed the proliferation, migration and invasion of PTC cells. Mechanistic analyses showed that HYOU1 silencing promoted oxidative phosphorylation while inhibited aerobic glycolysis via downregulating LDHB at the posttranscriptional level. We further confirmed that the 3'UTR of LDHB mRNA is the indirect target of HYOU1 silencing and HYOU1 silencing increased miR-375-3p levels. While LDHB overexpression significantly suppressed the inhibitory effects of HYOU1 silencing on aerobic glycolysis, proliferation, migration and invasion in PTC cells. Taken together, our findings suggest that HYOU1 promotes glycolysis and malignant progression in PTC cells via upregulating LDHB expression, providing a potential target for developing novel anticancer agents.

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