Expression of Immunoglobulin G in Human Proximal Tubular Epithelial Cells

Overview

Journal Mol Med Rep

Specialty Molecular Biology

Date 2021 Mar 24

PMID 33760139

Citations 7

Authors

Zhenling Deng

Ziyang Jing

Yanhong Guo

Junfan Ma

Huige Yan

Zhan Shi

Hui Deng

Yaoxian Liang

Song Wang

Zhuan Cui

Yuejuan Pan

Xiaoyan Qiu

Yue Wang

Affiliations

Soon will be listed here.

Abstract

Proximal tubular epithelial cells (PTECs) have innate immune characteristics, and produce proinﬂammatory factors, chemokines and complement components that drive epithelial‑mesenchymal transition (EMT). Our previous studies revealed that human mesangial cells and podocytes were able to synthesize and secrete immunoglobulin (Ig)A and IgG, respectively. The aim of the present study was to evaluate the expression of Igs in PTECs. Firstly, IgG was detected in the cytoplasm, the cell membrane and the lumen of PTECs in the normal renal cortex by immunohistochemistry. Secondly, Igγ gene transcription and V(D)J recombination were detected in single PTECs by nested PCR and Sanger sequencing. Thirdly, Igγ, Igκ and Igλ were clearly detected in an immortalized PTEC line (HK‑2) by immunostaining and western blotting, in which RP215 (an antibody that predominantly binds to non‑B cell‑derived IgG) was used. In addition, Igγ, Igκ and Igλ gene transcripts, conservative V(D)J recombination in the Igγ variable region, recombination activating gene 1/2 and activation‑induced cytidine deaminase were all detected in HK‑2 cells. These data suggested that PTECs may express IgG in a similar manner to B cells. Furthermore, IgG expression was upregulated by TGF‑β1 and may be involved in EMT.

Citing Articles

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