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Recreating Mouse Cortico-hippocampal Neuronal Circuit in Microfluidic Devices to Study BDNF Axonal Transport Upon Glucocorticoid Treatment

Overview
Journal STAR Protoc
Publisher Cell Press
Specialties Biology
Biomedical Engineering
Science
Date 2021 Mar 22
PMID 33748784
Citations 6
Authors
Sophie Lenoir
Aurelie Genoux
Fabienne Agasse
Frederic Saudou
Sandrine Humbert
Affiliations
Soon will be listed here.
Abstract

BDNF levels are reduced in the chronically stressed brain, in the area of hippocampus. Part of the hippocampal BDNF is provided by neuronal projection of the entorhinal cortex. Studying the cortico-hippocampal transport of BDNF is technically difficult. Here, we describe a protocol that reproduces mouse cortico-hippocampal circuit by plating neurons on the microfluidic devices and infecting the neurons with virus-encoding BDNF-mCherry, which allows investigation of the effects of elevated corticosterone levels on BDNF axonal transport. For complete details on the use and execution of this protocol, please refer to Agasse et al. (2020).

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