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SIRT6 Maintains Redox Homeostasis to Promote Porcine Oocyte Maturation

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Specialty Cell Biology
Date 2021 Mar 15
PMID 33718364
Citations 5
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Abstract

SIRT6, the sixth member of the sirtuin family proteins, has been characterized as a crucial regulator in multiple molecular pathways related to aging, including genome stability, DNA damage repair, telomere maintenance, and inflammation. However, the exact roles of SIRT6 during female germ cell development have not yet been fully determined. Here, we assessed the acquisition of meiotic competency of porcine oocytes by inhibition of SIRT6 activity. We observed that SIRT6 inhibition led to the oocyte meiotic defects by showing the impairment of polar body extrusion and cumulus cell expansion. Meanwhile, the compromised spindle/chromosome structure and actin dynamics were also present in SIRT6-inhibited oocytes. Moreover, SIRT6 inhibition resulted in the defective cytoplasmic maturation by displaying the disturbed distribution dynamics of cortical granules and their content ovastacin. Notably, we identified that transcript levels of genes related to oocyte meiosis, oxidative phosphorylation, and cellular senescence were remarkably altered in SIRT6-inhibited oocytes by transcriptome analysis and validated that the meiotic defects caused by SIRT6 inhibition might result from the excessive reactive oxygen species (ROS)-induced early apoptosis in oocytes. Taken together, our findings demonstrate that SIRT6 promotes the porcine oocyte meiotic maturation through maintaining the redox homeostasis.

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References
1.
Kanfi Y, Naiman S, Amir G, Peshti V, Zinman G, Nahum L . The sirtuin SIRT6 regulates lifespan in male mice. Nature. 2012; 483(7388):218-21. DOI: 10.1038/nature10815. View

2.
Pucci B, Villanova L, Sansone L, Pellegrini L, Tafani M, Carpi A . Sirtuins: the molecular basis of beneficial effects of physical activity. Intern Emerg Med. 2013; 8 Suppl 1:S23-5. DOI: 10.1007/s11739-013-0920-3. View

3.
Watroba M, Dudek I, Skoda M, Stangret A, Rzodkiewicz P, Szukiewicz D . Sirtuins, epigenetics and longevity. Ageing Res Rev. 2017; 40:11-19. DOI: 10.1016/j.arr.2017.08.001. View

4.
Finkel T, Deng C, Mostoslavsky R . Recent progress in the biology and physiology of sirtuins. Nature. 2009; 460(7255):587-91. PMC: 3727385. DOI: 10.1038/nature08197. View

5.
Tian X, Firsanov D, Zhang Z, Cheng Y, Luo L, Tombline G . SIRT6 Is Responsible for More Efficient DNA Double-Strand Break Repair in Long-Lived Species. Cell. 2019; 177(3):622-638.e22. PMC: 6499390. DOI: 10.1016/j.cell.2019.03.043. View